Publications

114) Yuzhen Feng, Dominik van Bodegraven, Alan Kádek, Ignacio L.B. Munguira, Laura Soria-Martinez, Sarah Nentwich, Sreedeepa Saha, Florian Chardon, Daniel Kavan, Charlotte Uetrecht, Mario Schelhaas, Wouter H. Roos*
Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility
Nature Communications, 2024, accepted

113) Richards, C. J., Melero Martinez, P., Roos, W. H. & Åberg, C.
High-Throughput Approach to Measure Number of Nanoparticles Associated with Cells: Size Dependence and Kinetic Parameters
Nanoscale Advances, 2024, in press

112) Sander de Weerd, Emma A. Ruiter, Eleonora Calicchia, Giuseppe Portale, Jan Jacob Schuringa, Wouter H. Roos, Anna Salvati
Optimization of Cell Membrane Purification for the Preparation and Characterization of Cell Membrane Liposomes
Small Methods, 2024, in press

111) Adéla Melcrova, Christiaan Klein, Wouter H. Roos*
Membrane-active antibiotics affect domains in bacterial membranes as the first step of their activity
Nano Letters, 2024, Vol. 24, 11800–11807
https://doi.org/10.1021/acs.nanolett.4c01873

110) Roberta Palmulli, Mickaël Couty, Melissa C. Piontek, Maharajah Ponnaiah, Florent Dingli, Frederik J. Verweij, Stéphanie Charrin, Matteo Tantucci, Sajitha Sasidharan, Eric Rubinstein, Anatol Kontush, Damarys Loew, Marie Lhomme, Wouter H. Roos, Graça Raposo, Guillaume van Niel
CD63 sorts cholesterol into endosomes for storage and distribution via exosomes
Nature Cell Biology, 2024, Vol. 26, 1093–1109
https://doi.org/10.1038/s41556-024-01432-9

109) Rafael B. Lira, Laura S. Dillingh, Jan-Jacob Schuringa, Gokhan Yahioglu, Klaus Suhling, Wouter H. Roos
Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes
Biophysical Journal, 2024, Vol. 123, p1592-1609
https://doi.org/10.1016/j.bpj.2024.04.033

108) Jekhmane, S., Derks, M.G.N., Maity, S., Slingerland, C.J.,, Tehrani, K.H.M.E., Medeiros-Silva, J., Vermeer, B., Ammerlaan, D., Charitou, V., Weijde, M., Elenbaas, B.O.W., Fetz, C., Cox, R., Lorent, J.H.F.F., Baldus, M., Künzler, M., Lelli, M., Cochrane, S., Martin, N.I., Roos, W.H., Breukink, E., Weingarth, M.
Host defense peptide plectasin targets bacterial cell wall precursor Lipid II by a calcium-sensitive supramolecular mechanism
Nature Microbiology, 2024, Vol. 9, 1778–1791
https://doi.org/10.1038/s41564-024-01696-9

107) Chris van Ewijk, Fan Xu, Sourav Maity, Jinyu Sheng, Marc. C. A. Stuart, Ben L. Feringa and Wouter H. Roos*
Light-Triggered Disassembly of Molecular Motor-based Supramolecular Polymers Revealed by High Speed-AFM 
Angewandte Chemie, 2024, Vol. 63, e202319387
https://doi.org/10.1002/anie.202319387

106) Harry Warner, Giulia Franciosa, Guus van der Borg, Britt Coenen, Felix Faas, Claire Koenig, Rinse de Boer, René Classens, Sjors Maassen, Maksim Baranov, Shweta Mahajan, Deepti Dabral, Frans Bianchi, Niek van Hilten, Herre Jelger Risselada, Wouter H. Roos, Jesper Olsen, Laia Querol Cano, Geert van den Bogaart
Atypical Cofilin Signaling Drives Dendritic Cell Migration through the Extracellular Matrix via Nuclear Deformation 
Cell Reports, 2024, Vol. 43, 113866
https://www.biorxiv.org/content/10.1101/2023.07.10.548429v1

105) Garima CN Thakur, Arunima Uday, Marek Cebecauer, Wouter H Roos, Lukasz Cwiklik, Martin Hof, Piotr Jurkiewicz, Adéla Melcrová
Charge of a transmembrane peptide alters its interaction with lipid membranes 
Colloids and Surfaces B: Biointerfaces, 2024, Vol. 235, 113765
https://doi.org/10.1016/j.colsurfb.2024.113765

104) van Ewijk C, Maity S, Roos WH*
Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy 
Methods Mol. Biol., 2024, Vol. 2694, p355-372
https://doi.org/10.1007/978-1-0716-3377-9_17

103) Feng Y, Roos WH*
Atomic Force Microscopy: An Introduction 
Methods Mol. Biol., 2024, Vol. 2694, p295-316
https://doi.org/10.1007/978-1-0716-3377-9_14

102) Liu K, Blokhuis AWP, van Ewijk C, Armin Kiani, Juntian Wu, Wouter H. Roos, Sijbren Otto
Light-Driven Eco-Evolutionary Dynamics in a Synthetic Replicator System 
Nature Chemistry, 2024, Vol. 16, p79-88
https://doi.org/10.1038/s41557-023-01301-2

101) Rafael B Lira, Jayna C F Hammond, Rafaela R M Cavalcanti, Madelief Rous, Karin A Riske, Wouter H Roos*
The underlying mechanical properties of membranes tune their ability to fuse 
J. Biol. Chem., 2023, Vol. 299, p105430
https://doi.org/10.1016/j.jbc.2023.105430

100) Richards, Ceri; Burgers, Thomas; Vlijm, Rifka; Roos, Wouter H.*; Åberg, Christoffer
Rapid internalization of nanoparticles by human cells at the single particle level 
ACS Nano, 2023, Vol. 17, Issue 17, p16287-16290
https://doi.org/10.1021/acsnano.3c01124

99) Pedro Buzón, Alejandro Velázquez-Cruz, Laura Corrales-Guerrero, Antonio Díaz-Quintana, Irene Díaz-Moreno, Wouter H. Roos*
The histone chaperones SET/TAF-1β and NPM1 exhibit conserved functionality in nucleosome remodelling and histone eviction in a cytochrome c-dependent manner 
Advanced Science, 2023, Vol. 10, Issue 29, Art. 2301859
https://doi.org/10.1002/advs.202301859

98) R. Shukla, A.J. Peoples, K.C. Ludwig, S. Maity, M.G.N. Derks, S. de Benedetti, A.M Krueger, B.J.A. Vermeulen, F. Lavore, R.V. Honorato, F. Grein, A. Bonvin, U. Kubitscheck, E. Breukink, C. Achorn, A. Nitti, C.J. Schwalen, A.L. Spoering, L.L. Ling, D. Hughes, M. Lelli, W.H. Roos, K. Lewis, T. Schneider, M. Weingarth
A new antibiotic from an uncultured bacterium binds to an immutable target 
Cell, 2023, Vol. 186, Issue 19, Art. e27, p4059-4073
https://doi.org/10.1016/j.cell.2023.07.038

97) Adéla Melcrová, Sourav Maity, Josef Melcr, Niels A. W. de Kok, Mariella Gabler, Jonne van der Eyden, Wenche Stensen, John S. M. Svendsen, Arnold J. M. Driessen, Siewert J. Marrink, Wouter H. Roos*
Lateral membrane organization as target of an antimicrobial peptidomimetic compound 
Nature Communications, 2023, Vol. 14, Issue 1, Art. 4038
https://doi.org/10.1038/s41467-023-39726-5

96) Y. Feng, R. Pogan, L. Thiede, J. Müller-Guhl, C. Uetrecht, W. H. Roos*
Fucose Binding Cancels out Mechanical Differences between Distinct Human Noroviruses 
Viruses, 2023, Vol. 15(7), Art. 1482
https://doi.org/10.3390/v15071482

95) Daphne Montizaan, Catherine Saunders, Keni Yang, Sajitha Sasidharan, Sourav Maity, Catharina Reker-Smit, Marc C. A. Stuart, Costanza Montis, Debora Berti, Wouter H. Roos, Anna Salvati
Role of curvature-sensing proteins in the uptake of nanoparticles with different mechanical properties 
Small, 2023, Vol. 19, Issue 39, p2303267
https://doi.org/10.1002/smll.202303267

94) Guus van der Borg, Niek Crone, Aimee L. Boyle, Alexander Kros and Wouter H. Roos*
SNARE mimic peptide triggered membrane fusion kinetics revealed using single particle techniques 
Physical Chemistry Chemical Physics, 2023, Vol. 25, Issue 18, p13019-13026
https://doi.org/10.1039/d2cp04448j

93) Guus van der Borg, Harry Warner, Melina Ioannidis, Geert van den Bogaart and Wouter H. Roos*
PLA 3D Printing as a Straightforward and Versatile Fabrication Method for PDMS Molds 
Polymers, 2023, Vol. 15, p1498  
https://doi.org/10.3390/polym15061498

92) Ceri J. Richards, Majid Ahmadi, Marc C. A. Stuart, Bart J. Kooi, Christoffer Åberg, Wouter H. Roos*
The effect of biomolecular corona on adsorption onto and desorption from a model lipid membrane 
Nanoscale, 2023, Vol. 15, p248-258 
https://doi.org/10.1039/D2NR05296B

91) Kimi Azad, Delphine Guilligay, Cecile Boscheron, Sourav Maity, Nicola De Franceschi, Guidenn Sulbaran, Gregory Effantin, Haiyan Wang, Jean-Philippe Kleman, Patricia Bassereau, Guy Schoehn, Wouter H Roos, Ambroise Desfosses, Winfried Weissenhorn
Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage 
Nature Structural & Molecular Biology, 2023, Vol. 30, p81-90
https://doi.org/10.1038/s41594-022-00867-8

90) Piontek, M. C., & Roos, W. H.*
Lipoprotein particles exhibit distinct mechanical properties 
Journal of Extracellular Biology, 2022, Vol. 1, e68
https://doi.org/10.1002/jex2.68

89) Wouter H. Roos
High speed AFM reveals the dynamics of virus budding 
Biophysical Journal, 2022, Vol. 121, p. 4022-4023
https://doi.org/10.1016/j.bpj.2022.09.027

88) R. Shukla, F. Lavore, S. Maity, M.G.N. Derks, C.R. Jones, B.J.A. Vermeulen, A. Melcrová, M.A. Morris, L. Marie Becker, X. Wang, R. Kumar, J. Medeiros-Silva, R.A.M. van Beekveld, A.M.J.J. Bonvin, J. Lorent, M. Lelli, J. Nowick, H.D. MacGillavry, A.J. Peoples, A.L. Spoering, L.L. Ling, D.E. Hughes, W.H. Roos, E. Breukink, K. Lewis, and M. Weingarth
Teixobactin kills bacteria by a two-pronged attack on the cell envelope 
Nature, 2022, Vol. 608, 390–396
https://doi.org/10.1038/s41586-022-05019-y

87) Alex van der Ham, Xue Liu, Dario Calvani, Adéla Melcrová, Melania Kozdra, Francesco Buda, Hermen Overkleeft, Wouter H. Roos, Dmitri Filippov, and Gregory Schneider
Freestanding Non-covalent Thin Films of the Propeller-shaped Polycyclic Aromatic Hydrocarbon Decacyclene 
Nature Communications, 2022, Vol. 13, 1920
https://doi.org/10.1038/s41467-022-29429-8

86) Karolina Majsterkiewicz, Artur P. Biela, Sourav Maity, Mohit Sharma, Bernard M. A. G. Piette, Agnieszka Kowalczyk, Szymon Gaweł, Soumyananda Chakraborti, Wouter H. Roos and Jonathan G. Heddle
Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
Nano Letters, 2022, Vol. 22, 3187–3195
https://doi.org/10.1021/acs.nanolett.1c04222

85) Sourav Maity, Gianluca Trinco, Pedro Buzón, Zaid R. Anshari, Noriyuki Kodera, Kien Xuan Ngo, Toshio Ando, Dirk J. Slotboom, Wouter H. Roos*
High Speed-Atomic Force Microscopy reveals a three state elevator-mechanism in the citrate transporter CitS 
Proc. Natl. Acad. Sci. U. S. A., 2022, 119 (6), e2113927119
https://doi.org/10.1073/pnas.2113927119

84) Pedro Buzón, Sourav Maity, Panagiotis Christodoulis, Monique J. Wiertsema, Steven Dunkelbarger, Christine Kim, Gijs J. Wuite, Adam Zlotnick & Wouter H. Roos*
Virus self-assembly proceeds through contact-rich energy minima
Science Advances, 2021, Vol. 7, eabg0811
https://doi.org/10.1126/sciadv.abg0811

83) Maryam Alqabandi, Nicola de Franceschi, Sourav Maity, Nolwenn Miguet, Marta Bally, Wouter H. Roos, Winfried Weissenhorn, Patricia Bassereau and Stéphanie Mangenot
The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization
BMC Biology, 2021, Vol. 19, Art. 66
https://doi.org/10.1186/s12915-021-00983-9

82) Gwenda F. Vasse, Pedro Buzón, Barbro N. Melgert, Wouter H. Roos*, Patrick van Rijn
Single Cell Reactomics: Real-time single-cell activation kinetics of optically trapped macrophages
Small Methods, 2021, 5, 2000849
https://doi.org/10.1002/smtd.202000849

81) Robijn F. Bruinsma, Gijs J. L. Wuite, Wouter H. Roos*
Physics of Viral Dynamics
Nature Reviews Physics, 2021, 3, 76–91
https://doi.org/10.1038/s42254-020-00267-1

80) M. Wenzel, I.N. Celik Gulsoy, Y. Gao, Z. Teng, J. Willemse, M. Middelkamp, M.G.M. van Rosmalen, P.W.B. Larsen, N.N. van der Wel, G.J.L. Wuite, W.H. Roos, L.W. Hamoen
Control of septum thickness by the curvature of SepF polymers
Proc. Natl. Acad. Sci. U. S. A., 2021, 118 (2) e2002635118
https://doi.org/10.1073/pnas.2002635118

79) Farkhad Maksudov, Olga Kononova, Aida Llauró, Alvaro Ortega-Esteban, Trevor Douglas, Gabriela N. Condenzo, Carmen San Martín, Kenneth A. Marx, Gijs J. L. Wuite, Wouter H. Roos, Pedro J. de Pablo, and Valeri Barsegov
Fluctuating Nonlinear Spring theory: strength, deformability, and toughness of biological nanoparticles from theoretical reconstruction of force-deformation spectra
Acta Biomaterialia, 2021, Vol. 122, 263-277
https://doi.org/10.1016/j.actbio.2020.12.043

78) Kai Liu, Jing Sun, Chao Ma, Sourav Maity, Fan Wang, Yu Zhou, Giuseppe Portale, Robert Göstl, Wouter H Roos, Hongjie Zhang, Andreas Herrmann
Reversibly photo‐modulating mechanical stiffness and toughness of bioengineered protein fibers
Angewandte Chemie, 2021, Vol. 60, Issue 6, 3222-3228
https://doi.org/10.1002/anie.202012848

77) S. Maity, J. Ottelé, G. Monreal Santiago, P. Frederix, P. Kroon, O. Markovitch, M. Stuart, S. Marrink, S. Otto, W. H. Roos*
Caught in the Act: Mechanistic insight into Supramolecular Polymerization driven Self-Replication from Real Time Visualization
Journal of the American Chemical Society, 2020, Vol. 142, Issue 32, 13709–13717
https://doi.org/10.1021/jacs.0c02635

76) Alejandro Valbuena, Sourav Maity, Mauricio Mateu, Wouter H. Roos*
Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
ACS Nano, 2020, Vol. 14, Issue 7, 8724–8734
https://doi.org/10.1021/acsnano.0c03207

75) Daan Vorselen, Melissa C. Piontek, Wouter H. Roos*, Gijs J. L. Wuite
Mechanical characterization of liposomes and extracellular vesicles, a protocol
Frontiers in Molecular Biosciences, 2020, Vol. 7, Art. 139
https://doi.org/10.3389/fmolb.2020.00139

74) Chao Ma, Jingjin Dong, Marco Viviani, Isotta Tulini, Nicola Pontillo, Sourav Maity, Yu Zhou, Wouter H. Roos, Kai Liu, Andreas Herrmann, Giuseppe Portale
De novo rational design of a freestanding, supercharged polypeptide, proton conducting membrane
Science Advances, 2020, Vol. 6, no. 29, eabc0810
https://doi.org/10.1126/sciadv.abc0810

73) Aurélie Bertin, Nicola De Franceschi, Eugenio De La Mora, Sourav Maity, Maryam Alqabandi, Nolwenn Miguet, Aurélie Di Cicco, Wouter H. Roos, Stéphanie Mangenot, Winfried Weissenhorn and Patricia Bassereau
Human ESCRT-III Polymers Assemble on Positively Curved Membranes and Induce Helical Membrane Tube Formation
Nature Communications, 2020, Vol. 11, Art. 2663
https://doi.org/10.1038/s41467-020-16368-5

72) L.G. Rikkert, P. Beekman, J. Caro, F.A.W. Coumans, A. Enciso Martinez, G. Jenster, S. le Gac, W. Lee, Ton G. van Leeuwen, G.B. Loozen, A. Nanou, R. Nieuwland, H.L. Offerhaus, C. Otto, D.M. Pegtel, M.C. Piontek, E . van der Pol, L. de Rond, W.H. Roos, R.B.M. Schasfoort, Marca Wauben, H . Zuilhof and Leon W.M.M. Terstappen
Cancer-ID: towards identification of cancer by tumor-derived extracellular vesicles in blood
Frontiers in Oncology, 2020, Vol. 10, Art. 608
https://doi.org/10.3389/fonc.2020.00608

71) Pedro Buzón, Sourav Maity, Wouter H. Roos*
Physical Virology: from Virus Self-assembly to Particle Mechanics
WIREs Nanomedicine & Nanobiotechnology, 2020, Vol. 12, Issue 4
https://doi.org/10.1002/wnan.1613

70) Mariska G. M. van Rosmalen, Douwe Kamsma, Andreas S. Biebricher, Chenglei Li, Adam Zlotnick, Wouter H. Roos* and Gijs J.L. Wuite*
Revealing in real-time a multi-step assembly mechanism for SV40 virus-like particles
Science Advances, 2020, Vol. 6, no. 16, eaaz1639
https://doi.org/10.1126/sciadv.aaz1639

69) R. Sorkin, M. Marchetti, E. Logtenberg, M. C. Piontek, E. Kerklingh, G. Brand, R. Voleti, J. Rizo, W. H. Roos, A. J. Groffen and G. J. L. Wuite
Synaptotagmin-1 and Doc2b exhibit distinct membrane remodeling mechanisms
Biophysical Journal, 2020, Vol. 118, Issue 3, 643-656
https://doi.org/10.1016/j.bpj.2019.12.021

68) Melissa Piontek, Rafael Lira, Wouter H. Roos*
Active probing of the mechanical properties of biological and synthetic vesicles
BBA General subjects, 2020, in the press
https://doi.org/10.1016/j.bbagen.2019.129486

67) Margherita Marchetti, Douwe Kamsma, Ernesto Cazares Vargas, Armando Hernandez García, Paul van der Schoot, Renko de Vries, Gijs J. L. Wuite, and Wouter H. Roos*
Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level
Nano Letters, 2019, Vol. 19, 5746−5753
https://doi.org/10.1021/acs.nanolett.9b02376

66) Christophe Caillat, Sourav Maity, Nolwenn Miguet, Wouter H. Roos, Winfried Weissenhorn
The role of VPS4 in ESCRT-III polymer remodeling
Biochemical Society Transactions, 2019, Vol. 47, 441-448
https://doi.org/10.1042/BST20180026

65) D. Denning, S. Bennett, T. Mullen, C. Moyer, D. Vorselen, G.J.L. Wuite, G. Nemerow, W.H. Roos
Maturation of adenovirus primes the protein nano-shell for successful endosomal escape
Nanoscale, 2019, Vol. 11, 4865-4869
https://doi.org/10.1039/c8nr10182e

64) Sourav Maity, Christophe Caillat, Nolwenn Miguet, Guidenn Sulbaran, Gregory Effantin, Guy Schoehn, Wouter H. Roos* and Winfried Weissenhorn*
VPS4 triggers constriction and cleavage of ESCRT-III helical filaments
Science Advances, 2019, Vol. 5, eaau7198
https://doi.org/10.1126/sciadv.aau7198

63) Michael Krieg, Gotthold Fläschner, David Alsteens, Benjamin M. Gaub, Wouter H. Roos, Gijs J. L. Wuite, Hermann E. Gaub, Christoph Gerber, Yves F. Dufrêne, Daniel J. Müller
Atomic Force Microscopy-based Mechanobiology
Nature Reviews Physics, 2019, Vol. 1, 41–57
https://doi.org/10.1038/s42254-018-0001-7

62) Y. Guo, W. H. Roos*
AFM Nanoindentation Experiments on Protein Shells: A Protocol
Methods in Molecular Biology, 2018, Vol. 1886, 243-257
https://doi.org/10.1007/978-1-4939-8894-5_14

61) D. Vorselen, S. M. van Dommelen, R. Sorkin, M. C. Piontek, J. Schiller, S. T. Döpp, S. A. A. Kooijmans, B. A. van Oirschot, B. A. Versluijs, M. B. Bierings, R. van Wijk, R. M. Schiffelers, G. J. L. Wuite and W. H. Roos*
The Fluid Membrane determines Mechanics of Erythrocyte Extracellular Vesicles and is Softened in Hereditary Spherocytosis
Nature Communications, 2018, Vol. 9, 4960
https://doi.org/10.1038/s41467-018-07445-x

60) Mariska G.M. van Rosmalen, Chenglei Li, Adam Zlotnick, Gijs J.L. Wuite and Wouter H. Roos*
Effect of dsDNA on the assembly pathway and mechanical strength of SV40-VP1 virus-like particles
Biophysical Journal, 2018, Vol. 115, 1656-1665
https://doi.org/10.1016/j.bpj.2018.07.044

59) Raya Sorkin, Rick Huisjes, Filip Bošković, Daan Vorselen, Silvia Pignatelli, Yifat Ofir-Birin, Joames K. Freitas Leal, Jürgen Schiller, Wouter H. Roos, Giel Bosman, Neta Regev-Rudzki, Raymond M. Schiffelers, Gijs J. L. Wuite
Nanomechanics of Extracellular Vesicles Reveals Vesiculation Pathways
Small, 2018, Vol. 14, 1801650
https://doi.org/10.1002/smll.201801650

58) Raya Sorkin, Giulia Bergamaschi, Douwe Kamsma, Guy Brand, Elya Dekel, Yifat Ofir-Birin, Ariel Rudik, Marta Gironella, Felix Ritort, Neta Regev-Rudzki, Wouter H. Roos, and Gijs J.L Wuite
Probing cellular mechanics with Acoustic Force Spectroscopy
Molecular Biology of the Cell, 2018, Vol. 29, 1917-2035
https://doi.org/10.1091/mbc.E18-03-0154

57) Marek Cieplak and Wouter H. Roos*
Special issue on the physics of viral capsids
Journal of Physics-Condensed Matter, 2018, Vol. 30, 290201
https://doi.org/10.1088/1361-648X/aacb6c

56) Guus van der Borg, Scarlett Braddock, Jelle S. Blijleven, Antoine M. van Oijen and Wouter H. Roos*
Single particle fusion of influenza viruses reveals complex interactions with target membranes
Journal of Physics-Condensed Matter, 2018, Vol. 30, 204005
https://doi.org/10.1088/1361-648X/aabc21

55) Graeme A. King, Maryam Hashemi Shabestari, Kees-Karel H. Taris, Ashutosh K. Pandey, Sundararajan Venkatesh, Jayapalraja Thilagavathi, Kamalendra Singh, Rama Krishna Koppisetti, Dmitry Temiakov, Wouter H. Roos, Carolyn K. Suzuki, Gijs J. L. Wuite
Acetylation and phosphorylation of human TFAM regulate TFAM-DNA interactions via contrasting mechanisms
Nucleic Acids Research , 2018, Vol. 46, 3633-3642
https://doi.org/10.1093/nar/gky204

54) Sander Boonstra, Jelle S. Blijleven, Wouter H. Roos, Patrick R. Onck, Erik van der Giessen and Antoine M. van Oijen
Hemagglutinin-Mediated Membrane Fusion: A Biophysical Perspective
Annual Review of Biophysics, 2018, Vol. 47, 153-173
https://doi.org/10.1146/annurev-biophys-070317-033018

53) Daan Vorselen, Margherita Marchetti, Carmen López-Iglesias, Peter J. Peters, Wouter H. Roos* and Gijs J. L. Wuite
Multilamellar nanovesicles show distinct mechanical properties depending on their degree of lamellarity
Nanoscale, 2018, Vol. 10, 5318-5324
https://doi.org/10.1039/c7nr09224e

52) Mariska G. M. van Rosmalen, Glen R. Nemerow, Gijs J.L. Wuite and Wouter H. Roos*
A single point mutation in precursor protein VI doubles the mechanical strength of human adenovirus
Journal of Biological Physics, 2018, Vol. 44, 119-132
https://doi.org/10.1007/s10867-017-9479-y

51) W.H. Roos*
AFM nanoindentation of protein shells, expanding the approach beyond viruses
Seminars Cell & Develop Biol, 2018, Vol. 73, 145-152
https://doi.org/10.1016/j.semcdb.2017.07.044

50) Y. Liang, R.A. van der Valk, R.T. Dame, W.H. Roos, G.J.L. Wuite
Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling
Scientific Reports, 2017, Vol. 7, 15275
https://doi.org/10.1038/s41598-017-15477-4

49) L. Zhang, S. Maity, K. Liu, Q. Liu, R. Göstl, G. Portale, W. H. Roos, and A. Herrmann
Nematic DNA Thermotropic Liquid Crystals with Photoresponsive Mechanical Properties
Small, 2017, Vol. 13, 1701207
https://doi.org/10.1002/smll.201701207

48) J. Snijder, K. Radtke, F. Anderson, L. Scholtes, E. Corradini, J. Baines, A.J.R. Heck, G.J.L. Wuite, B. Sodeik, W.H. Roos*
Vertex-Specific Proteins pUL17 and pUL25 Mechanically Reinforce Herpes Simplex Virus Capsids
J. Virol., 2017, Vol. 91, e00123-17
https://doi.org/10.1128/JVI.00123-17

47) M. C. Piontek, W. H. Roos*
Atomic Force Microscopy: An Introduction
Methods Mol. Biol. , 2017, Vol. 1665, 243-258
https://doi.org/10.1007/978-1-4939-7271-5_13

46) D. Vorselen, F.C. MacKintosh, W.H. Roos*, G.J.L. Wuite
Competition between bending and internal pressure governs the mechanics of fluid nanovesicles
ACS Nano , 2017, Vol. 11, 2628-2636
https://doi.org/10.1021/acsnano.6b07302

45) M. Hashemi Shabestari, A.E.C. Meijering, W.H. Roos, G.J.L. Wuite and E.J.G. Peterman
Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy
Methods Enzymol. , 2017, Vol. 582, 85-119
https://doi.org/10.1016/bs.mie.2016.09.047

44)   D. Vorselen, E. S. Kooreman, G.J.L. Wuite, W. H. Roos*
Controlled tip wear on high roughness surfaces yields gradual broadening and rounding of cantilever tips
Sci Reports 2016, Vol. 6, 36972
https://doi.org/10.1038/srep36972

43 ) J. Snijder, O. Kononova, I. Barbu, C. Uetrecht, W. Rurup, R. Rose, M. S. Koay, J. J. Cornellissen, W. H. Roos, V. Barsegov, G. J. Wuite, A. J. Heck
Assembly and mechanical properties of the cargo-free and cargo-loaded bacterial nanocompartment encapsulin
Biomacromolecules, 2016, Vol. 17, 2522-2529
https://doi.org/10.1021/acs.biomac.6b00469

42) K. Heinze, E. Sasaki, N.P. King, D. Baker, D. Hilvert, G.J.L. Wuite, W.H. Roos*
Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM
J Phys Chem B , 2016, Vol. 120, 5945-5952
https://doi.org/10.1021/acs.jpcb.6b01464

41)   M. Baclayon, P. van Ulsen, H Mouhib, MH Shabestari, T Verzijden, S. Abeln, W. H. Roos, G. J. Wuite
Mechanical Unfolding of an Autotransporter Passenger Protein Reveals the Secretion Starting Point and Processive Transport Intermediates
ACS Nano , 2016, Vol. 10, 5710-5719
https://doi.org/10.1021/acsnano.5b07072

40)   M. Marchetti, G. J. Wuite, W. H. Roos*
Atomic Force Microscopy Observation and Characterization of Single Virions and Virus-Like Particles by Nano-Indentation
Current Opinion in Virology , 2016, Vol. 18, 82-88
https://doi.org/10.1016/j.coviro.2016.05.002

39)   D. Denning and W. H. Roos*
Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches
Cell Adhesion & Migration, 2016, Vol. 10, 540-553
https://doi.org/10.1080/19336918.2016.1170259

38)   O. Kononova, J. Snijder, Y. Kholodov, K. A. Marx, G. J. Wuite, W. H. Roos*, V. Barsegov
Fluctuating Nonlinear Spring Model of Mechanical Deformation of Biological Particles.
PLoS Computational Biology, 2016, Vol. 12, e1004729
https://doi.org/10.1371/journal.pcbi.1004729

37)   R. Ayadi, W. H. Roos
Building an artificial actin cortex
Methods Cell Biol. , 2015, Vol. 128, 105-124
https://doi.org/10.1016/bs.mcb.2015.01.012

36)   Y. Liang, U. Li Chen, M. van Rosmalen, G. J. Wuite & W. H. Roos*
Sample Preparation and Imaging of Single Adenovirus Particle Using Atomic Force Microscopy in Liquid
Chinese Journal of Virology , 2015, 31, 601-6

35)   M. G. M. van Rosmalen, W. H. Roos, G. J. L. Wuite
Material properties of Viral Nanocages explored by Atomic Force Microscopy
Methods Mol. Biol. , 2015, Vol. 1252, 115-137
https://doi.org/10.1007/978-1-4939-2131-7_11

34)   G. Farge, M. Mehmedovic, M. Baclayon, S. van den Wildenberg, W. H. Roos, C. Gustafsson, G. J. L. Wuite, M. Falkenberg
In vitro reconstituted nucleoids can block mitochondrial DNA replication and transcription
Cell Reports, 2014, Vol. 8, 66-74
https://doi.org/10.1016/j.celrep.2014.05.046

33)   D. Vorselen, W. H. Roos, F. C. MacKintosh, G. J. L. Wuite, J. J. W. A. van Loon
The role of the cytoskeleton in sensing changes in gravity by non-specialized cells
FASEB J., 2014, Vol. 28, 536-547
https://doi.org/10.1096/fj.13-236356

32)   D. Luque, A. de la Escosura, J. Snijder, M. Brasch, R. Rose, M. Koay, J. Carrascosa, G. J. Wuite, W. H. Roos, A. J. Heck, J. Cornelissen, T. Torres, J. Castón
Self-Assembly and Characterization of Small and Monodisperse Dye Nanospheres in a Protein Cage
Chemical Science, 2014, Vol. 5, 575-581
https://doi.org/10.1039/C3SC52276H

31)   O. Kononova, J. Snijder, M. Brasch, J. Cornelissen, R. I. Dima, K. A. Marx, G. J. Wuite, W. H. Roos*, V. Barsegov
Structural Transitions and Energy Landscape for Cowpea Chlorotic Mottle Virus Capsid mechanics from nanomanipulation in vitro and in silico
Biophys. J., 2013, Vol. 105, 1893-1903
See also New and Notable: Capsid deformations reveal complex mechano-chemistry
https://doi.org/10.1016/j.bpj.2013.08.032

30)   J. Snijder, C. Uetrecht, R. Rose, R. Sanchez-Eugenia, G. Marti, J. Agirre, D. Guérin, G. Wuite, A. J. R. Heck, W. H. Roos*
Probing the biophysical interplay between a viral genome and its capsid
Nature Chemistry, 2013, Vol. 5, 502-509
See also Nature Chemistry News and Views: Biophysical chemistry: Unravelling capsid transformations
https://doi.org/10.1038/nchem.1627

29)   J. Snijder, V. S. Reddy, E. R. May, W. H. Roos*, G. R. Nemerow, G. J. L. Wuite
Integrin and defensin modulate the mechanical properties of adenovirus
J. Virol., 2013, Vol. 87, 2756-2766
See also JVI Spotlight: Host Factors involved in uncoating modulate Adenovirus elasticity
https://doi.org/10.1128/JVI.02516-12

28)   W. S. Klug, W. H. Roos*, G. J. L. Wuite
Unlocking internal pre-stress from protein nanoshells
Phys. Rev. Lett., 2012, Vol. 109, 168104
See also Physics: Protein Shells Take a Strength Test
See also NRC Handelsblad: Virusomhulsel dankt zijn stevigheid aan zijn hoekjes (27 Oct 2012)
https://doi.org/10.1103/PhysRevLett.109.168104

27)   J. Snijder, I. Ivanovska, M. Baclayon, W. H. Roos*, G. J. L. Wuite
Probing the impact of loading rate on the mechanical properties of viral nanoparticles
Micron, 2012, Vol. 43, 1343-1350
https://doi.org/10.1016/j.micron.2012.04.011

26)   D. Heuveling, G. Visser, M. de Groot, J. F. de Boer, M. Baclayon, W. H. Roos, G. Wuite, C. Leemans, R. de Bree, G. A. M. S. van Dongen
Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node
Eur. J. Nucl. Med. Mol. Imaging, 2012, Vol. 39, 1161-1168
https://doi.org/10.1007/s00259-012-2080-5

25)   W. H. Roos, I. Gertsman, E. May, C. Brooks III, J. Johnson, G. J. Wuite
Mechanics of bacteriophage maturation
Proc. Natl. A cad. Sci. U. S. A., 2012, Vol. 109, 2342-2347
https://doi.org/10.1073/pnas.1109590109

24)   M. Baclayon, G. K. Shoemaker, C. Uetrecht, S. Crawford, M. K. Estes, B. Prasad, A. J. Heck, G. J. Wuite, W. H. Roos*
Pre-stress strenghtens the shell of Norwalk Virus Nanoparticles
Nano Letters, 2011, Vol. 11, 4865-4869
https://doi.org/10.1021/nl202699r

23)   W. H. Roos
How to perf orm a nanoindentation experiment on a virus
Methods Mol. Biol. , 2011, Vol. 783, 251-264
https://doi.org/10.1007/978-1-61779-282-3_14

22)   D. Heuveling, G. Visser, M. Baclayon, W. H. Roos, G. J. Wuite, O. Hoekstra, C. Leemans, R. de Bree, G. van Dongen
89 Zr-Nanocolloidal Albumin–Based PET/CT Lymphoscintigraphy for Sentinel Node Detection in Head and Neck Cancer: Preclinical Results
J. Nuclear Medicine , 2011, Vol. 52, 1580-1584
https://doi.org/10.2967/jnumed.111.089557

21)   A. Evilevitch, W. H. Roos, I. L. Ivanovska, M. Jeembaeva, B. Jönsson, G. J. L. Wuite
Effects of salts on internal DNA pressure and mechanical properties of phage capsids
J. Mol. Biology, 2011, Vol. 405, 18-23
https://doi.org/10.1016/j.jmb.2010.10.039

20)   W. H. Roos*, R. Bruinsma, G. J. L. Wuite
Physical Virology
Nature Physics, 2010, Vol. 6, 733-743
https://doi.org/10.1038/nphys1797

19)   M. Baclayon, G. J. L. Wuite, W. H. Roos*
Imaging and manipulation of single viruses by atomic force microscopy
Soft Matter, 2010, Vol. 6, 5273-5285
https://doi.org/10.1039/B923992H

18)   W. H. Roos, M. M. Gibbons, A. Arkhipov, C. Uetrecht, N. Watts, P Wingfield, A. C. Steven, A. Heck, K. Schulten, W. Klug, G. J. L. Wuite
Squeezing protein shells: how continuum elastic models, molecular dynamics simulations and experiments coalesce at the nanoscale
Biophysical J., 2010, Vol. 99, 1175-1181
Featuring as a Biophysical Journal Research Highlight
https://doi.org/10.1016/j.bpj.2010.05.033

17)   G. K. Shoemaker, E. van Duijn, S. Crawford, C. Uetrecht, M. Baclayon, W. H. Roos, G. Wuite, M. Estes, B. Venkataram Prasad, A. J. R. Heck
Norwalk virus assembly and stability monitored by mass spectrometry
Mol. & Cell. Proteomics, 2010, Vol. 9, 1742-1751
https://doi.org/10.1074/mcp.M900620-MCP200

16)   M. Baclayon, W. H. Roos*, G. J. L. Wuite
Sampling protein form and function with the atomic force microscope
Mol. & Cell. Proteomics, 2010, Vol. 9, 1678-1688
https://doi.org/10.1074/mcp.R110.001461

15)   W. H. Roos and G. J. L. Wuite
Mechanical Properties of viruses
Emerging Topics in Physical Virology, 2010, Eds. Stockley & Twarock, 85-102
https://doi.org/10.1016/j.virusres.2012.06.008

14)   R. J. Kroeze, M. N. Helder, W. H. Roos, G. J. L. Wuite, R. A. Bank, T. H. Smit
The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells
Acta Biomaterialia, 2010, Vol. 6, 2060-2065
https://doi.org/10.1016/j.actbio.2009.11.022

13)   A. Arkhipov, W. H. Roos, G. Wuite, K. Schulten
Elucidating the mechanism behind irreversible deformation of viral capsids
Biophys J., 2009, Vol. 97, 2061-2069
https://doi.org/10.1016/j.bpj.2009.07.039

12)   W. H. Roos, K. Radtke, E. Kniesmeijer, H. Geertsema, B. Sodeik, G. J. L. Wuite
Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids
Proc. Natl. Acad. Sci. U. S. A. 2009, Vol. 106, 9673-9678
See also Kennislink: VU kraakt mechanische structuur herpesvirus
See also Science Daily: Punching holes into herpes viruses
https://doi.org/10.1073/pnas.0901514106

11)   T. Haraszti, S. Schulz, K. Uhrig, R. Kurre, W. Roos, C. Schmitz, J. Curtis, T. Maier, A. Clemen, J. Spatz
Biomimetic models of the actin cortex
Biophys. Rev. & Lett., 2009, Vol. 4, 17-32
https://doi.org/10.1142/S1793048009001009

10)   W. H. Roos*, G. J. L. Wuite
Nanoindentation studies reveal material properties of viruses
Adv. Mat., 2009, Vol. 21, 1187-1192
https://doi.org/10.1002/adma.200801709

9)     W. H. Roos, O. Campas, F. Montel, G. Woehlke, J. P. Spatz , P. Bassereau, G. Cappello
Dynamic kinesin-1 clustering on microtubules due to mutually attractive interactions
Phys. Biol. 2008, Vol. 5, 046004
https://doi.org/10.1088/1478-3975/5/4/046004

8)     C. Uetrecht, C. Versluis, N. R. Watts, W. H. Roos, G. J. Wuite, P. T. Wingfield, A. C. Steven, A. J. Heck
High-resolution mass spectrometry of viral assemblies: Molecular composition and stability of dimorphic hepatitis B virus capsids
Proc. Natl. Acad. Sci. U. S. A., 2008, Vol. 105, 9216-9220
See also Volkskrant: Opbouw hepatitis B virus in kaart gebracht (28 juli 2008)
https://doi.org/10.1073/pnas.0800406105

7)     W. H. Roos, I. L. Ivanovska, A. Evilevitch, G. J. L. Wuite
Viral capsids: mechanical characteristics, genome packaging and delivery mechanisms
Cell. Mol. Life Sci., 2007, Vol. 64, 1484-1497
https://doi.org/10.1007/s00018-007-6451-1

6)     C. Mohrdieck, F. Dalmas, E. Arzt, R. Tharmann, M. M. A. E. Claessens, A. R. Bausch, C. Schmitz, J. Curtis, W. Roos, S. Schulz, K. Uhrig, A. Roth, E. Sackmann, J. P. Spatz
Biomimetic Models of the Actin Cytoskeleton
Small, 2007, Vol. 3, 1015-1022
https://doi.org/10.1002/smll.200600565

5)     V. K. Kodali, W. Roos, J. P. Spatz, J. E. Curtis
Cell-assisted assembly of colloidal crystallites
Soft Matter, 2007, Vol. 3, 337-348
See also: Cell structure made crystal clear? (2007) Chem Biol 2: B11
https://doi.org/10.1039/B611022N

4)     W. Roos, J. Ulmer, S. Gräter, T. Surrey, J. P. Spatz
Microtubule Gliding and Cross-Linked Microtubule Networks on Micropillar Interfaces
Nano Letters 2005, Vol. 5, 2630-2634
https://doi.org/10.1021/nl051865j

3)     C. Mohrdieck, A. Wanner, W. Roos, A. Roth, E. Sackmann, J.P. Spatz, E. Arzt
A Theoretical Description of Elastic Pillar Substrates in Biophysical Experiments
ChemPhysChem 2005, Vol. 6, 1492-1498
https://doi.org/10.1002/cphc.200500109

2)     W. H. Roos, A. Roth, J. Konle, H. Presting, E. Sackmann, J. P. Spatz
Freely Suspended Actin Cortex Models on Arrays of Microfabricated Pillars
ChemPhysChem 2003, Vol. 4, 872-877
https://doi.org/10.1002/cphc.200300712

1)     M. Haupt, A. Ladenburger, R. Sauer, K. Thonke, R. Glass, W. Roos, J. P. Spatz, H. Rauscher, S. Riethmüller, M. Möller
Ultraviolet-emitting ZnO nanowhiskers prepared by a vapor transport process on prestructured surfaces with self-assembled polymers
J. Applied Physics 2003, Vol. 93, 6252-6257
https://doi.org/10.1063/1.1563845