Section Systems Medicine of Metabolism and Signalling

The Section Systems Medicine of Metabolism and Signalling studies the regulation of energy metabolism and mTOR (mammalian target of rapamycin) signalling in healthy and disease states. Energy metabolism and the mTOR signalling network are often deregulated in metabolic diseases, cancer and ageing. The section aims to unravel the mechanisms behind the disease states and thereby be able to predict severity of disease symptoms and new drug targets. In order to achieve this we adopt cell biology, biochemistry, proteomics, and systems biology approaches.

Expertise

Cell biology:

• Immunoblot, immunofluorescence, immunoprecipitation
• Cell culture
• Proliferation and apoptosis assays (Incucyte)

Biochemistry:

• Enzyme activity
• ¹³ C metabolic flux analysis
• Metabolomics
• Oxygen consumption flux analysis (Oroboros; SeaHorse)

Proteomics:

• Relative proteomics (Orbitrap)
• Targeted quantitative proteomics
• Phosphoproteomics

Systems Biology:

• Detailed kinetic models
• Genome-scale models
• Metabolic Control Analysis and Hierarchical Regulation Analysis
• Biological thermodynamics