Skip to ContentSkip to Navigation
Research GBB Membrane Enzymology Publications

Publications

2025

Presutti, C., Vreeker, E., Sasidharan, S., Ferdinando, Z., Stuart, M., Juhaniewicz-Dębińska, J., Maglia, G., Roos, W. H., & Poolman, B. (2025). Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-b-PEO Block Copolymers. Biomacromolecules, Article 4c01651. Advance online publication. https://doi.org/10.1021/acs.biomac.4c01651
Karapanagioti, F., Obermaier, S., Slotboom, D. J., & Poolman, B. (2025). The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum. FEBS Letters. Advance online publication. https://doi.org/10.1002/1873-3468.70044
Pérez Carrillo, V. H., Whittaker, J. J., Wiedemann, C., Harder, J.-M., Lohr, T., Jamithireddy, A. K., Dajka, M., Goretzki, B., Joseph, B., Guskov, A., Harmer, N. J., Holzgrabe, U., & Hellmich, U. A. (2025). Structure and Dynamics of Macrophage Infectivity Potentiator Proteins from Pathogenic Bacteria and Protozoans Bound to Fluorinated Pipecolic Acid Inhibitors. Journal of Medicinal Chemistry, 68(5), 5926-5941. https://doi.org/10.1021/acs.jmedchem.5c00134

2024

Heinen, L., van den Noort, M., King, M. S., Kunji, E. R. S., & Poolman, B. (2025). Synthetic syntrophy for adenine nucleotide cross-feeding between metabolically active nanoreactors. Nature Nanotechnology, 20, 112-120. https://doi.org/10.1038/s41565-024-01811-1
Foster, A. J., van den Noort, M., & Poolman, B. (2024). Bacterial cell volume regulation and the importance of cyclic di-AMP. Microbiology and Molecular Biology Reviews, 88(2), Article e0018123. https://doi.org/10.1128/mmbr.00181-23
Patiño-Ruiz, M. F., Anshari, Z. R., Gaastra, B., Slotboom, D. J., & Poolman, B. (2024). Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation. Nature Communications, 15(1), Article 7976. https://doi.org/10.1038/s41467-024-52085-z
Coenradij, J., Bailoni, E., & Poolman, B. (2024). Construction of Out-of-Equilibrium Metabolic Networks in Nano- and Micrometer-Sized Vesicles. Journal of visualized experiments : JoVE, (206), Article 66627. https://doi.org/10.3791/66627
Linnik, D., Maslov, I., Punter, C. M., & Poolman, B. (2024). Dynamic structure of E. coli cytoplasm: supramolecular complexes and cell aging impact spatial distribution and mobility of proteins. Communications biology, 7(1), Article 508. https://doi.org/10.1038/s42003-024-06216-3
Szomek, M., Akkerman, V., Lauritsen, L., Walther, H.-L., Juhl, A. D., Thaysen, K., Egebjerg, J. M., Covey, D. F., Lehmann, M., Wessig, P., Foster, A. J., Poolman, B., Werner, S., Schneider, G., Müller, P., & Wüstner, D. (2024). Ergosterol promotes aggregation of natamycin in the yeast plasma membrane. Biochimica et Biophysica Acta - Biomembranes, 1866(7), Article 184350. https://doi.org/10.1016/j.bbamem.2024.184350
Nemchinova, M., Schuurman-Wolters, G. K., Whittaker, J. J., Arkhipova, V., Marrink, S. J., Poolman, B., & Guskov, A. (2024). Exploring the Ligand Binding and Conformational Dynamics of the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ. The Journal of Physical Chemistry B, 128(32), 7822-7832. https://doi.org/10.1021/acs.jpcb.4c02662
Karapanagioti, F., Atlason, Ú. Á., Slotboom, D. J., Poolman, B., & Obermaier, S. (2024). Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters. eLife, 13, Article RP93971. https://doi.org/10.7554/eLife.93971
Monterroso, B., Margolin, W., Boersma, A. J., Rivas, G., Poolman, B., & Zorrilla, S. (2024). Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions. Chemical reviews, 124(4), 1899–1949. Article 3c00622. https://doi.org/10.1021/acs.chemrev.3c00622
Foster, A. J., Li, H., Drougkas, P., Schuurman-Wolters, G. K., ten Kate, J., Paulino, C., & Poolman, B. (2024). Membrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP. Communications biology, 7(1), Article 1710. https://doi.org/10.1038/s42003-024-07420-x
van der Sleen, L., Stevens, J. A., Marrink, S. J., Poolman, B., & Tych, K. (2024). Probing the stability and interdomain interactions in the ABC transporter OpuA using single-molecule optical tweezers. Cell reports, 43(4), Article 114110. https://doi.org/10.1016/j.celrep.2024.114110
Bailoni, E., Patiño-Ruiz, M. F., Stan, A. R., Schuurman-Wolters, G. K., Exterkate, M., Driessen, A. J. M., & Poolman, B. (2024). Synthetic Vesicles for Sustainable Energy Recycling and Delivery of Building Blocks for Lipid Biosynthesis. ACS Synthetic Biology, 13(5), 1549–1561. https://doi.org/10.1021/acssynbio.4c00073
Iyer, A., Frallicciardi, J., le Paige, U. B. A., Narasimhan, S., Luo, Y., Alvarez Sieiro, P., Syga, L., van den Brekel, F., Minh Tran, B., Tjioe, R., Schuurman-Wolters, G., C A Stuart, M., Baldus, M., van Ingen, H., & Poolman, B. (2024). The structure and function of the bacterial osmotically inducible protein Y. Journal of Molecular Biology, 436(16), Article 168668. https://doi.org/10.1016/j.jmb.2024.168668
van den Noort, M., Drougkas, P., Paulino, C., & Poolman, B. (2024). The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact. eLife, 12, Article 90996. https://doi.org/10.7554/eLife.90996
The Atomwise AIMS Program, Wallach, I., Bernard, D., Nguyen, K., Ho, G., Morrison, A., Stecula, A., Rosnik, A., Garaeva, A. A., Borowska, A., & Slotboom, D. J. (2024). AI is a viable alternative to high throughput screening: a 318-target study. Scientific Reports, 14(1), Article 7526. https://doi.org/10.1038/s41598-024-54655-z
Diamanti, E., Souza, P. C. T., Setyawati, I., Bousis, S., Monjas, L., Swier, L. J. Y. M., Shams, A., Tsarenko, A., Stanek, W. K., Jäger, M., Marrink, S. J., Slotboom, D. J., & Hirsch, A. K. H. (2024). Author Correction: Identification of inhibitors targeting the energy-coupling factor (ECF) transporters. Communications biology, 7, Article 70. https://doi.org/10.1038/s42003-024-05770-0
Nijland, M., Lefebvre, S. N., Thangaratnarajah, C., & Slotboom, D. J. (2024). Bidirectional ATP-driven transport of cobalamin by the mycobacterial ABC transporter BacA. Nature Communications, 15(1), Article 2626. https://doi.org/10.1038/s41467-024-46917-1
Martínez Felices, J. M., Barreto, Y. B., Thangaratnarajah, C., Whittaker, J. J., Alencar, A. M., Guskov, A., & Slotboom, D. J. (2024). Cobalamin decyanation by the membrane transporter BtuM. Structure, 32. https://doi.org/10.1016/j.str.2024.04.014
Shams, A., Bousis, S., Diamanti, E., Elgaher, W. A. M., Zeimetz, L., Haupenthal, J., Slotboom, D. J., & Hirsch, A. K. H. (2024). Expression and characterization of pantothenate energy-coupling factor transporters as an anti-infective drug target. Protein Science, 33(11), Article e5195. https://doi.org/10.1002/pro.5195
Exapicheidou, I. A., Shams, A., Ibrahim, H., Tsarenko, A., Backenköhler, M., Hamed, M. M., Diamanti, E., Volkamer, A., Slotboom, D. J., & Hirsch, A. K. H. (2024). Hit optimization by dynamic combinatorial chemistry on Streptococcus pneumoniae energy-coupling factor transporter ECF-PanT. Chemical communications (Cambridge, England), 60, 870–873. https://doi.org/10.1039/d3cc04738e
Borowska, A. M., Chiariello, M. G., Garaeva, A. A., Rheinberger, J., Marrink, S. J., Paulino, C., & Slotboom, D. J. (2024). Structural basis of the obligatory exchange mode of human neutral amino acid transporter ASCT2. Nature Communications, 15(1), Article 6570. https://doi.org/10.1038/s41467-024-50888-8
Partipilo, M., & Jan Slotboom, D. (2024). The S-component fold: a link between bacterial transporters and receptors. Communications biology, 7(1), Article 610. https://doi.org/10.1038/s42003-024-06295-2
Bergsma, T., Steen, A., Kamenz, J. L., Otto, T., Gallardo, P., & Veenhoff, L. M. (2025). Imaging-Based Quantitative Assessment of Biomolecular Condensates in vitro and in Cells. The Journal of Biological Chemistry, 301(2), Article 108130. https://doi.org/10.1016/j.jbc.2024.108130
Lyu, Y. X., Fu, Q., Wilczok, D., Ying, K., King, A., Antebi, A., Vojta, A., Stolzing, A., Moskalev, A., Georgievskaya, A., Maier, A. B., Olsen, A., Groth, A., Simon, A. K., Brunet, A., Jamil, A., Kulaga, A., Bhatti, A., Yaden, B., ... Bakula, D. (2024). Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity. Aging, 16(20), 12955-12976. https://doi.org/10.18632/aging.206135
Otto, T. A., Bergsma, T., Dekker, M., Mouton, S. N., Gallardo, P., Wolters, J. C., Steen, A., Onck, P. R., & Veenhoff, L. M. (2024). Nucleoporin Nsp1 surveils the phase state of FG-Nups. Cell reports, 43(10), Article 114793. https://doi.org/10.1016/j.celrep.2024.114793
Terpstra, H. M., Gómez-Sánchez, R., Veldsink, A. C., Otto, T. A., Veenhoff, L. M., & Heinemann, M. (2024). PunctaFinder: An algorithm for automated spot detection in fluorescence microscopy images. Molecular Biology of the Cell, 35(12), Article 35:mr9. https://doi.org/10.1091/mbc.E24-06-0254
Molina-Sánchez, M. D., Martínez-Abarca, F., Millán, V., Mestre, M. R., Stehantsev, P., Stetsenko, A., Guskov, A., & Toro, N. (2024). Adaptive immunity of type VI CRISPR-Cas systems associated with reverse transcriptase-Cas1 fusion proteins. Nucleic Acids Research, 52(22), 14229-14243. Article gkae1154. https://doi.org/10.1093/nar/gkae1154
Podoliak, E., Lamm, G. H. U., Marin, E., Schellbach, A. V., Fedotov, D. A., Stetsenko, A., Asido, M., Maliar, N., Bourenkov, G., Balandin, T., Baeken, C., Astashkin, R., Schneider, T. R., Bateman, A., Wachtveitl, J., Schapiro, I., Busskamp, V., Guskov, A., Gordeliy, V., ... Kovalev, K. (2024). A subgroup of light-driven sodium pumps with an additional Schiff base counterion. Nature Communications, 15, Article 3119. https://doi.org/10.1038/s41467-024-47469-0
Sauciuc, A., Whittaker, J., Tadema, M., Tych, K., Guskov, A., & Maglia, G. (2024). Blobs form during the single-file transport of proteins across nanopores. Proceedings of the National Academy of Sciences, 121(38), Article e2405018121. https://doi.org/10.1073/pnas.2405018121
Lohr, T., Herbst, C., Bzdyl, N. M., Jenkins, C., Scheuplein, N. J., Sugiarto, W. O., Whittaker, J. J., Guskov, A., Norville, I., Hellmich, U. A., Hausch, F., Sarkar-Tyson, M., Sotriffer, C., & Holzgrabe, U. (2024). High Affinity Inhibitors of the Macrophage Infectivity Potentiator Protein from Trypanosoma cruzi, Burkholderia pseudomallei, and Legionella pneumophila: A Comparison. ACS Infectious Diseases, 10(10), 3681–3691. https://doi.org/10.1021/acsinfecdis.4c00553
Torres, S. D. C., Castro-Amarante, M. F., Ferreira, L. C. S., Guskov, A., & Balan, A. (2024). Successful production of antibodies against extra cytoplasmic loops of the Mycobacterium tuberculosis ABC transporter Rv1819c. Preprints. https://doi.org/10.20944/preprints202404.0481.v1
Sauciuc, A., Whittaker, J., Tadema, M., Tych, K., Guskov, A., & Maglia, G. (2024). Unravelled proteins form blobs during translocation across nanopores. (bioRxiv). https://doi.org/10.1101/2024.01.23.576815

2023

Minh Tran, B., Michiel Punter, C., Linnik, D., Iyer, A., & Poolman, B. (2024). Single-protein diffusion in the periplasm of Escherichia coli. Journal of Molecular Biology, 436(4), Article 168420. https://doi.org/10.1016/j.jmb.2023.168420
Bailoni, E., Partipilo, M., Coenradij, J., Grundel, D. A. J., Slotboom, D. J., & Poolman, B. (2023). Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective. ACS Synthetic Biology, 12. https://doi.org/10.1021/acssynbio.3c00062
Poolman, B. (2023). Physicochemical homeostasis in bacteria. FEMS Microbiology Reviews, 47(4), Article fuad033. https://doi.org/10.1093/femsre/fuad033
Mantovanelli, L., Linnik, D. S., Punter, M., Kojakhmetov, H. J., Śmigiel, W. M., & Poolman, B. (2023). Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli. PLoS Computational Biology, 19(9), Article e1011093. https://doi.org/10.1371/journal.pcbi.1011093
Tran, B. M., Linnik, D. S., Punter, C. M., Śmigiel, W. M., Mantovanelli, L., Iyer, A., O’Byrne, C., Abee, T., Johansson, J., & Poolman, B. (2023). Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes. Communications biology, 6(1), Article 51. https://doi.org/10.1038/s42003-023-04423-y
Partipilo, M., Claassens, N. J., & Slotboom, D. J. (2023). A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells. ACS Synthetic Biology, 12, 947-962. Article 3c00070. https://doi.org/10.1021/acssynbio.3c00070
Partipilo, M., Whittaker, J. J., Pontillo, N., Coenradij, J., Herrmann, A., Guskov, A., & Slotboom, D. J. (2023). Biochemical and structural insight into the chemical resistance and cofactor specificity of the formate dehydrogenase from Starkeya novella. The FEBS Journal, 290(17), 4238-4255. https://doi.org/10.1111/febs.16871
Thangaratnarajah, C., Nijland, M., Borges-Araújo, L., Jeucken, A., Rheinberger, J., Marrink, S. J., Souza, P. C. T., Paulino, C., & Slotboom, D. J. (2023). Expulsion mechanism of the substrate-translocating subunit in ECF transporters. Nature Communications, 14(1), Article 4484. https://doi.org/10.1038/s41467-023-40266-1
Diamanti, E., Souza, P. C. T., Setyawati, I., Bousis, S., Gómez, L. M., Swier, L. J. Y. M., Shams, A., Tsarenko, A., Stanek, W. K., Jäger, M., Marrink, S. J., Slotboom, D. J., & Hirsch, A. K. H. (2023). Identification of inhibitors targeting the energy-coupling factor (ECF) transporters. Communications biology, 6(1), Article 1182. https://doi.org/10.1038/s42003-023-05555-x
Colucci, E., Anshari, Z., Patiño-Ruiz, M., Nemchinova, M., Whittaker, J., Slotboom, D., & Guskov, A. (2023). Mutation in glutamate transporter homologue GltTk provides insights into pathologic mechanism of episodic ataxia 6. Nature Communications, 14, Article 1799. https://doi.org/10.1038/s41467-023-37503-y
van der Zouwen, A. J., Jeucken, A., van der Pol, E., Boerema, G., Slotboom, D. J., & Witte, M. D. (2023). The linkage-type and the exchange molecule affect the protein-labeling efficiency of iminoboronate probes. Organic & Biomolecular Chemistry, 21, 9173-9181. https://doi.org/10.1039/d3ob01269g
Mouton, S. N., Boersma, A. J., & Veenhoff, L. M. (2023). A physicochemical perspective on cellular ageing. Trends in Biochemical Sciences, 48(11), 949-962. https://doi.org/10.1016/j.tibs.2023.08.007
Barrientos, E. C. R., Otto, T. A., Mouton, S. N., Steen, A., & Veenhoff, L. M. (2023). A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport. Nucleus, 14(1), Article 2240139. https://doi.org/10.1080/19491034.2023.2240139
Veldsink, A. C., Gallardo, P., Lusk, C. P., & Veenhoff, L. M. (2023). Changing the guard—nuclear pore complex quality control. FEBS Letters, 597(22), 2739-2749. https://doi.org/10.1002/1873-3468.14739
Kozai, T., Fernandez-Martinez, J., van Eeuwen, T., Gallardo, P., Kapinos, L. E., Mazur, A., Zhang, W., Tempkin, J., Panatala, R., Delgado-Izquierdo, M., Raveh, B., Sali, A., Chait, B. T., Veenhoff, L. M., Rout, M. P., & Lim, R. Y. H. (2023). Dynamic molecular mechanism of the nuclear pore complex permeability barrier. BioRxiv. https://doi.org/10.1101/2023.03.31.535055
Veldsink, A. C., & Veenhoff, L. M. (2023). How to unravel a basket: NPC reorganization during meiosis. Journal of Cell Biology, 222(2), Article e202301044. https://doi.org/10.1083/jcb.202301044
Marin, E., Kovalev, K., Poelman, T., Veenstra, R., Borshchevskiy, V., & Guskov, A. (2024). Custom Design of a Humidifier Chamber for In Meso Crystallization. Crystal Growth & Design, 24(1), 325-330. Article 3c01034. https://doi.org/10.1021/acs.cgd.3c01034
Marin, E., Kovaleva, M., Kadukova, M., Mustafin, K., Khorn, P., Rogachev, A., Mishin, A., Guskov, A., & Borshchevskiy, V. (2024). Regression-Based Active Learning for Accessible Acceleration of Ultra-Large Library Docking. Journal of chemical information and modeling, 64(7), 2612–2623. Article 3c01661. https://doi.org/10.1021/acs.jcim.3c01661
Wiedemann, C., Whittaker, J. J., Carrillo, V. H. P., Goretzki, B., Dajka, M., Tebbe, F., Harder, J.-M., Krajczy, P. R., Joseph, B., Hausch, F., Guskov, A., & Hellmich, U. A. (2023). Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding. International Journal of Biological Macromolecules, 252, Article 126366. https://doi.org/10.1016/j.ijbiomac.2023.126366
Kolosova , O., Zgadzay, Y., Stetsenko, A., Atamas, A., Wu, C.-H., Sachs, M. S., Jenner, L., Guskov, A., & Yusupov, M. (2023). Structural characterization of cephaeline binding to the eukaryotic ribosome using Cryo-Electron Microscopy. Biopolymers and Cell, 39(4), 265-276. https://doi.org/10.7124/bc.000AA4
Atamas, A. A., Guskov, A. I., & Rogachev, A. V. (2023). Structural Study of the Candida auris Ribosome. Moscow University Biological Sciences Bulletin, 78(Suppl 1), S56-S58. https://doi.org/10.3103/S0096392523700220
Bikmullin, A. G., Fatkhullin, B., Stetsenko, A., Gabdulkhakov, A., Garaeva, N., Nurullina, L., Klochkova, E., Golubev, A., Khusainov, I., Trachtmann, N., Blokhin, D., Guskov, A., Validov, S., Usachev, K., & Yusupov, M. (2023). Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from S. aureus. International Journal of Molecular Sciences, 24(3), Article 24032118. https://doi.org/10.3390/ijms24032118

2022

Bailoni, E., & Poolman, B. (2022). ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis. ACS Synthetic Biology, 11(7), 2348–2360. Article 2c00075. https://doi.org/10.1021/acssynbio.2c00075
Frallicciardi, J., Gabba, M., & Poolman, B. (2022). Determining small-molecule permeation through lipid membranes. Nature protocols, 17, 2620-2646. https://doi.org/10.1038/s41596-022-00734-2
Frallicciardi, J., Melcr, J., Siginou, P., Marrink, S. J., & Poolman, B. (2022). Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes. Nature Communications, 13(1), Article 1605. https://doi.org/10.1038/s41467-022-29272-x
Szomek, M., Reinholdt, P., Walther, H. L., Scheidt, H. A., Müller, P., Obermaier, S., Poolman, B., Kongsted, J., & Wüstner, D. (2022). Natamycin sequesters ergosterol and interferes with substrate transport by the lysine transporter Lyp1 from yeast. Biochimica et Biophysica Acta - Biomembranes, 1864(11), Article 184012. https://doi.org/10.1016/j.bbamem.2022.184012
Smigiel, W. M., Mantovanelli, L., Linnik, D. S., Punter, M., Silberberg, J., Xiang, L., Xu, K., & Poolman, B. (2022). Protein diffusion in Escherichia coli cytoplasm scales with the mass ofthe complexes and is location dependent. Science Advances, 8(32), Article eabo5387. https://doi.org/10.1126/sciadv.abo5387
Aarts, N., Bovenberg, R., Dogterom, M., van Est, R., Gerritsen, J., Macnaghten, P., Poolman, B., Robaey, Z., Rasmussen, S., Ruivenkamp, G., Thole, E., Tremmel, G., van der Vlugt, C., & Zwart, H. (2022). Society and synthetic cells: A position paper by the Future Panel on Synthetic Life. Rathenau Instituut.
Partipilo, M., Yang, G., Mascotti, L., Slotboom, D., & Fraaije, M. (2022). A conserved sequence motif in the E. coli soluble FAD-containing pyridine nucleotide transhydrogenase is important for reaction efficiency. The Journal of Biological Chemistry, 298(9), Article 102304. https://doi.org/10.1016/j.jbc.2022.102304
Walter, J. D., Scherer, M., Hutter, C. A. J., Garaeva, A. A., Zimmermann, I., Wyss, M., Rheinberger, J., Ruedin, Y., Earp, J. C., Egloff, P., Sorgenfrei, M., Hürlimann, L. M., Gonda, I., Meier, G., Remm, S., Thavarasah, S., van Geest, G., Bruggmann, R., Zimmer, G., ... Seeger, M. A. (2022). Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance. Embo Reports, 23(4), Article e54199. https://doi.org/10.15252/embr.202154199
Zhou, W., Trinco, G., Slotboom, D. J., Forrest, L. R., & Faraldo-Gómez, J. D. (2022). Correction to: On the Role of a Conserved Methionine in the Na+-Coupling Mechanism of a Neurotransmitter Transporter Homolog (Neurochemical Research, (2022), 47, 1, (163-175), 10.1007/s11064-021-03253-w). NEUROCHEMICAL RESEARCH, 47(1), 176. https://doi.org/10.1007/s11064-021-03420-z
Maity, S., Trinco, G., Buzón, P., Anshari, Z. R., Kodera, N., Ngo, K. X., Ando, T., Slotboom, D. J., & Roos, W. H. (2022). High-speed atomic force microscopy reveals a three-state elevator mechanism in the citrate transporter CitS. Proceedings of the National Academy of Sciences of the United States of America, 119(6), Article e2113927119. https://doi.org/10.1073/pnas.2113927119
Nijland, M., Martínez Felices, J. M., Slotboom, D. J., & Thangaratnarajah, C. (2022). Membrane transport of cobalamin. In G. Litwack (Ed.), Vitamins and Hormones (pp. 121-148). (Vitamins and Hormones; Vol. 119). ACADEMIC PRESS INC ELSEVIER SCIENCE. https://doi.org/10.1016/bs.vh.2022.01.008
Wan, Q., Mouton, S. N., Veenhoff, L. M., & Boersma, A. J. (2022). A FRET-based method for monitoring structural transitions in protein self-organization. Cell Reports: Methods, 2(3), 100184. https://doi.org/10.1016/j.crmeth.2022.100184
Kuiper, E. F. E., Gallardo, P., Bergsma, T., Mari, M., Kolbe Musskopf, M., Kuipers, J., Giepmans, B. N. G., Steen, A., Kampinga, H. H., Veenhoff, L. M., & Bergink, S. (2022). The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis. Nature Cell Biology, 24, 1584-1594. https://doi.org/10.1038/s41556-022-01010-x
Pijning, T., Te Poele, E. M., de Leeuw, T. C., Guskov, A., & Dijkhuizen, L. (2022). Crystal Structure of 4,6-α-Glucanotransferase GtfC-ΔC from Thermophilic Geobacillus 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes. Journal of Agricultural and Food Chemistry, 70(48), 15283–15295. Article 2c06394. https://doi.org/10.1021/acs.jafc.2c06394
Xu, W., Ni, D., Hou, X., Pijning, T., Guskov, A., Rao, Y., & Mu, W. (2022). Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity. Journal of Agricultural and Food Chemistry, 70(16), 5095-5105. https://doi.org/10.1021/acs.jafc.2c01225
Zgadzay, Y., Kolosova , O., Stetsenko, A., Wu, C., Bruchlen, D., Usachev, K., Validov, S., Jenner, L., Rogachev, A., Yusupova, G., Sachs, M. S., Guskov, A., & Yusupov, M. (2022). E-site drug specificity of the human pathogen Candida albicans ribosome. Science Advances, 8(21), Article eabn1062. https://doi.org/10.1126/sciadv.abn1062

2021

Iyer, A., Baranov, M., Foster, A. J., Chordia, S., Roelfes, G., Vlijm, R., van den Bogaart, G., & Poolman, B. (2021). Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy. ACS chemical biology, 16(5), 891-904. Article acschembio.1c00100. https://doi.org/10.1021/acschembio.1c00100
Mantovanelli, L., Gaastra, B. F., & Poolman, B. (2021). Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell. In M. A. Model, & I. Levitan (Eds.), Current Topics in Membranes: New Methods and Sensors for Membrane and Cell Volume Research (Vol. 88, pp. 1-54). (Current Topics in Membranes). ACADEMIC PRESS INC ELSEVIER SCIENCE. https://doi.org/10.1016/bs.ctm.2021.10.002
Åberg, C., & Poolman, B. (2021). Glass-Like Characteristics of Intracellular Motion in Human Cells. Biophysical Journal, 120(11), 2355-2366. https://doi.org/10.1016/j.bpj.2021.04.011
Alvarez-Sieiro, P., Sikkema, H. R., & Poolman, B. (2021). Heterodimer Formation of the Homodimeric ABC Transporter OpuA. International Journal of Molecular Sciences, 22(11), Article 5912. https://doi.org/10.3390/ijms22115912
Sikkema, H. R., & Poolman, B. (2021). In silico method for selecting residue pairs for single-molecule microscopy and spectroscopy. Scientific Reports, 11(1), Article 5756. https://doi.org/10.1038/s41598-021-85003-0
Tran, B. M., Prabha, H., Iyer, A., O'Byrne, C., Abee, T., & Poolman, B. (2021). Measurement of Protein Mobility in Listeria monocytogenes Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion. Frontiers in Microbiology, 12, Article 640149. https://doi.org/10.3389/fmicb.2021.640149
Partipilo, M., Ewins, E. J., Frallicciardi, J., Robinson, T., Poolman, B., & Slotboom, D. J. (2021). Minimal Pathway for the Regeneration of Redox Cofactors. JACS Au, 1(12), 2280-2293. https://doi.org/10.1021/jacsau.1c00406
van den Noort, M., de Boer, M., & Poolman, B. (2021). Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein. Journal of Molecular Biology, 433(15), Article 167036. https://doi.org/10.1016/j.jmb.2021.167036
Plötz, E., Schuurman-Wolters, G., Zijlstra, N., Jager, A., Griffith, D., Guskov, A., Gouridis, G., Poolman, B., & Cordes, T. (2021). Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ. Open Biology, 11(4), Article 200406. https://doi.org/10.1098/rsob.200406
Zhou, W., Trinco, G., Slotboom, D. J., Forrest, L. R., & Faraldo-Gómez, J. D. (2022). On the Role of a Conserved Methionine in the Na+-Coupling Mechanism of a Neurotransmitter Transporter Homolog. NEUROCHEMICAL RESEARCH, 47(1), 163-175. https://doi.org/10.1007/s11064-021-03253-w
Thangaratnarajah, C., Rheinberger, J., Paulino, C., & Slotboom, D. J. (2021). Insights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM. Proceedings of the National Academy of Sciences of the United States of America, 118(34), Article e2105014118. https://doi.org/10.1073/pnas.2105014118
Trinco, G., Arkhipova, V., Garaeva, A., Hutter, C. A. J., Seeger, M. A., Guskov, A., & Slotboom, D. (2021). Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk. Communications biology, 4, Article 751. https://doi.org/10.1038/s42003-021-02267-y
Garibsingh, R.-A. A., Ndaru, E., Garaeva, A. A., Shi, Y., Zielewicz, L., Zakrepine, P., Bonomi, M., Slotboom, D. J., Paulino, C., Grewer, C., & Schlessinger, A. (2021). Rational design of ASCT2 inhibitors using an integrated experimental-computational approach. Proceedings of the National Academy of Sciences of the United States of America, 118(37), Article e2104093118. https://doi.org/10.1073/pnas.2104093118
Arkhipova, V., Fu, H., Hoorens, M., Trinco, G., Lameijer, L., Marin, E., Feringa, B. L., Poelarends, G., Szymanski, W., Slotboom, D., & Guskov, A. (2021). Structural Aspects of Photopharmacology: Insight into the Binding of Photoswitchable and Photocaged Inhibitors to the Glutamate Transporter Homologue. Journal of the American Chemical Society, 143(3), 1513-1520. https://doi.org/10.1021/jacs.0c11336
Wan, Q., Mouton, S. N., Veenhoff, L. M., & Boersma, A. J. (2021). A precise and general FRET-based method for monitoring structural transitions in protein self-organization. European biophysics journal with biophysics letters, 50(SUPPL 1), 198.
De Vries, H., Fragasso, A., Otto, T., Klughammer, N., Andersson, J., Sluis, E. V. D., Steen, A., Dahlin, A., Veenhoff, L., Dekker, C., Giessen, E. V. D., & Onck, P. (2021). De Novo Computational Design of Disordered Fg-Nucleoporins. Biophysical Journal, 120(3), 29a-30a. https://doi.org/10.1016/j.bpj.2020.11.435
Semmelink, M. F. W., Steen, A., & Veenhoff, L. M. (2021). Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease-The Example of C9orf72 ALS. International Journal of Molecular Sciences, 22(17), Article 9217. https://doi.org/10.3390/ijms22179217
Semchonok, D. A., Mondal, J., Cooper, C. J., Schlum, K., Li, M., Amin, M., Sorzano, C. O. S., Ramírez-Aportela, E., Kastritis, P. L., Boekema, E. J., Guskov, A., & Bruce, B. D. (2022). Cryo-EM structure of a tetrameric photosystem I from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium. Plant Communications, 3(1), Article 100248. https://doi.org/10.1016/j.xplc.2021.100248
Stehantsev, P., Stetsenko, A., Nemchinova, M., Aduri, N. G., Marrink, S., Gati, C., & Guskov, A. (2021). A structural view onto disease-linked mutations in the human neutral amino acid exchanger ASCT1. Computational and Structural Biotechnology Journal, 19, 5246-5254. https://doi.org/10.1016/j.csbj.2021.09.015
Nemchinova, M., Melcr, J., Wassenaar, T., Marrink, S., & Guskov, A. (2021). Asymmetric CorA Gating Mechanism as Observed by Molecular Dynamics Simulations. Journal of chemical information and modeling, 61(5), 2407-2417. https://doi.org/10.1021/acs.jcim.1c00261
Volkova, M., Atamas, A., Tsarenko, A., Rogachev, A., & Guskov, A. (2021). Cation Transporters of Candida albicans—New Targets to Fight Candidiasis? Biomolecules, 11(4), Article 584. https://doi.org/10.3390/biom11040584
Stetsenko, A., Stehantsev, P., Dranenko, N. O., Gelfand, M. S., & Guskov, A. (2021). Structural and biochemical characterization of a novel ZntB (CmaX) transporter protein from Pseudomonas aeruginosa. International Journal of Biological Macromolecules, 184, 760-767. https://doi.org/10.1016/j.ijbiomac.2021.06.130

2020

Henderson, R. K., de Valk, S. C., Poolman, B., & Mans, R. (2021). Energy coupling of membrane transport and efficiency of sucrose dissimilation in yeast. Metabolic Engineering, 65, 243-254. https://doi.org/10.1016/j.ymben.2020.11.014
Pols, T., Singh, S., Deelman-Driessen, C., Gaastra, B. F., & Poolman, B. (2021). Enzymology of the pathway for ATP production by arginine breakdown. The FEBS Journal, 288(1), 293-309. Article febs.15337. https://doi.org/10.1111/febs.15337
Liu, B., Mavrova, S. N., van den Berg, J., Kristensen, S. K., Mantovanelli, L., Veenhoff, L. M., Poolman, B., & Boersma, A. J. (2020). Erratum. ACS Sensors, 5(5), 1500-1500. https://doi.org/10.1021/acssensors.0c00744
Van't Klooster, J. S., Bianchi, F., Doorn, R. B., Lorenzon, M., Lusseveld, J. H., Punter, C. M., & Poolman, B. (2020). Extracellular loops matter: Subcellular location and function of the lysine transporter Lyp1 from Saccharomyces cerevisiae. The FEBS Journal, 287(20), 4401-4414. Article febs.15262. https://doi.org/10.1111/febs.15262
Sikkema, H. R., van den Noort, M., Rheinberger, J., de Boer, M., Krepel, S. T., Schuurman-Wolters, G. K., Paulino, C., & Poolman, B. (2020). Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA. Science Advances, 6(47), Article eabd7697. https://doi.org/10.1126/sciadv.abd7697
Ruiz, S. J., van 't Klooster, J. S., Bianchi, F., & Poolman, B. (2020). Growth Inhibition by Amino Acids in Saccharomyces cerevisiae. Microorganisms, 9(1), 1-17. Article 9010007. https://doi.org/10.3390/microorganisms9010007
de Boer, M., Cordes, T., & Poolman, B. (2020). Kinetic Modelling of Transport Inhibition by Substrates in ABC Importers. Journal of Molecular Biology, 432(20), 5565-5576. https://doi.org/10.1016/j.jmb.2020.08.008
van 't Klooster, J. S., Cheng, T.-Y., Sikkema, H. R., Jeucken, A., Moody, D. B., & Poolman, B. (2020). Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae. Journal of Molecular Biology, 432(14), 4023-4031. https://doi.org/10.1016/j.jmb.2020.04.029
van 't Klooster, J. S., Cheng, T.-Y., Sikkema, H. R., Jeucken, A., Moody, B., & Poolman, B. (2020). Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins. eLife, 9, Article e57003. https://doi.org/10.7554/eLife.57003
Marinus, N., Tahiri, N., Duca, M., Mouthaan, L. M. C. M., Bianca, S., van den Noort, M., Poolman, B., Witte, M. D., & Minnaard, A. J. (2020). Stereoselective Protection-Free Modification of 3-Keto-saccharides. Organic letters, 22(14), 5622-5626. https://doi.org/10.1021/acs.orglett.0c01986
Thomas, C., Aller, S. G., Beis, K., Carpenter, E. P., Chang, G., Chen, L., Dassa, E., Dean, M., Duong Van Hoa, F., Ekiert, D., Ford, R., Gaudet, R., Gong, X., Holland, I. B., Huang, Y., Kahne, D. K., Kato, H., Koronakis, V., Koth, C. M., ... Tampé, R. (2020). Structural and functional diversity calls for a new classification of ABC transporters. FEBS Letters, 594(23), 3767-3775. Article 1873-3468.13935. https://doi.org/10.1002/1873-3468.13935
van der Zouwen, A. J. N., Jeucken, A., Steneker, R., Hohmann, K. F., Lohse, J., Slotboom, D. J., & Witte, M. (2021). Iminoboronates as Dual-Purpose Linkers in Chemical Probe Development. Chemistry, 27(10), 3292-3296. https://doi.org/10.1002/chem.202005115
Rempel, S., Gati, C., Nijland, M., Thangaratnarajah, C., Karyolaimos, A., Gier, J.-W. L. D., Guskov, A., & Slotboom, D. (2020). A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds. Nature, 580(7803), 409-412. https://doi.org/10.1038/s41586-020-2072-8
Jochim, A., Adolf, L., Belikova, D., Schilling, N. A., Setyawati, I., Chin, D., Meyers, S., Verhamme, P., Heinrichs, D. E., Slotboom, D. J., & Heilbronner, S. (2020). An ECF-type transporter scavenges heme to overcome iron-limitation in Staphylococcus lugdunensis. eLife, 9, Article e57322. https://doi.org/10.7554/eLife.57322
Slotboom, D. J., Ettema, T. W., Nijland, M., & Thangaratnarajah, C. (2020). Bacterial multi-solute transporters. FEBS Letters, 594(23), 3898-3907. https://doi.org/10.1002/1873-3468.13912
Garaeva, A. A., & Slotboom, D. J. (2020). Elevator-type mechanisms of membrane transport. Biochemical Society Transactions, 48(3), 1227-1241. Article BST20200290. https://doi.org/10.1042/BST20200290
Walter, J. D., Hutter, C. A., Garaeva, A. A., Scherer, M., Zimmermann, I., Wyss, M., Rheinberger, J., Ruedin, Y., Earp, J. C., Egloff, P., Sorgenfrei, M., Hürlimann, L. M., Gonda, I., Meier, G., Remme, S., Thavarasah, S., van der Geest, G., Bruggman, R., Zimmer, G., ... Seeger, M. A. (2020). Highly potent bispecific sybodies neutralize SARS-CoV-2. BioRxiv. https://doi.org/10.1101/2020.11.10.376822
Setyawati, I., Stanek, W. K., Dosz-Majsnerowska, M., Ziel-Swier, L., Pardon, E., Steyaert, J., Guskov, A., & Slotboom, D. (2020). In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters. eLife, 9, 1-21. Article e64389. https://doi.org/10.7554/eLife.64389
Faustino, I., Abdizadeh, H., Souza, P. C. T., Jeucken, A., Stanek, W. K., Guskov, A., Slotboom, D., & Marrink, S. (2020). Membrane mediated toppling mechanism of the folate energy coupling factor transporter. Nature Communications, 11(1), Article 1763. https://doi.org/10.1038/s41467-020-15554-9
Stetsenko, A., Singh, R., Jähme, M., Guskov, A., & Slotboom, D. (2020). Structural and Functional Characterization of NadR from Lactococcus lactis. Molecules, 25(8), Article 1940. https://doi.org/10.3390/molecules25081940
Arkhipova, V., Guskov, A., & Slotboom, D. (2020). Structural ensemble of a glutamate transporter homologue in lipid nanodisc environment. Nature Communications, 11(1), Article 998. https://doi.org/10.1038/s41467-020-14834-8
Mouton, S. N., Thaller, D. J., Crane, M. M., Rempel, I. L., Terpstra, O. T., Steen, A., Kaeberlein, M., Lusk, C. P., Boersma, A. J., & Veenhoff, L. M. (2020). A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast. eLife, 9, 1-23. Article 54707. https://doi.org/10.7554/eLife.54707
Mouton, S., Veenhoff, L. M., & Boersma, A. J. (2020). Macromolecular Crowding Measurements with Genetically Encoded Probes Based on Förster Resonance Energy Transfer in Living Cells. Methods in Molecular Biology, 2175, 169-180. https://doi.org/10.1007/978-1-0716-0763-3_12
Rempel, I. L., Steen, A., & Veenhoff, L. M. (2020). Poor old pores-The challenge of making and maintaining nuclear pore complexes in aging. The FEBS Journal, 287(6), 1058-1075. https://doi.org/10.1111/febs.15205
Stetsenko, A., & Guskov, A. (2020). Cation permeability in CorA family of proteins. Scientific Reports, 10(1), Article 840. https://doi.org/10.1038/s41598-020-57869-z
Skitchenko, R. K., Usoltsev, D., Uspenskaya, M., Kajava, A. V., & Guskov, A. (2020). Census of halide-binding sites in protein structures. Bioinformatics (Oxford, England), 36(10), 3064-3071. https://doi.org/10.1093/bioinformatics/btaa079
Dubach, V. R. A., & Guskov, A. (2020). The Resolution in X-ray Crystallography and Single-Particle Cryogenic Electron Microscopy. Crystals, 10(7), 1-13. Article 580. https://doi.org/10.3390/cryst10070580

2019

Syga, L., De Vries, R. H., van Oosterhout, H., Bartelds, R., Boersma, A. J., Roelfes, G., & Poolman, B. (2020). A trifunctional linker for palmitoylation and peptide and protein localization in biological membranes. ChemBioChem, 21(9), 1320-1328. https://doi.org/10.1002/cbic.201900655
Rempel, I. L., Popken, P., Ghavami, A., Mishra, A., Hapsari, R. A., Wolters, A. H. G., Veldsink, A. C., Klaassens, M., Meinema, A. C., Poolman, B., Giepmans, B. N. G., Onck, P. R., Steen, A., & Veenhoff, L. M. (2020). Flexible and Extended Linker Domains Support Efficient Targeting of Heh2 to the Inner Nuclear Membrane. Structure, 28(2), 185-195.e5. https://doi.org/10.1016/j.str.2019.11.003
Gabba, M., & Poolman, B. (2020). Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift. Biophysical Journal, 118(2), 435-447. Article bpj.2019.11.3383. https://doi.org/10.1016/j.bpj.2019.11.3383
Gabba, M., Frallicciardi, J., van 't Klooster, J., Henderson, R., Syga, Ł., Mans, R., van Maris, A. J. A., & Poolman, B. (2020). Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane. Biophysical Journal, 118(2), 422-434. https://doi.org/10.1016/j.bpj.2019.11.3384
Price, C. E., Branco Dos Santos, F., Hesseling, A., Uusitalo, J. J., Bachmann, H., Benavente, V., Goel, A., Berkhout, J., Bruggeman, F. J., Marrink, S.-J., Montalban-Lopez, M., de Jong, A., Kok, J., Molenaar, D., Poolman, B., Teusink, B., & Kuipers, O. P. (2019). Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength. BMC Evolutionary Biology, 19(1), Article 15. https://doi.org/10.1186/s12862-018-1331-x
Pols, T., Sikkema, H. R., Gaastra, B. F., Frallicciardi, J., Śmigiel, W. M., Singh, S., & Poolman, B. (2019). A synthetic metabolic network for physicochemical homeostasis. Nature Communications, 10(1), Article 4239. https://doi.org/10.1038/s41467-019-12287-2
Sikkema, H. R., Gaastra, B. F., Pols, T., & Poolman, B. (2019). Cell fueling and metabolic energy conservation in synthetic cells. ChemBioChem, 20(20 SI), 2581–2592. https://doi.org/10.1002/cbic.201900398
de Boer, M., Gkouridis, G., Vietrov, R., Begg, S. L., Schuurman-Wolters, G. K., Husada, F., Eleftheriadis, N., Poolman, B., McDevitt, C. A., & Cordes, T. (2019). Conformational and dynamical plasticity in substrate-binding proteins underlies selective transport in ABC importers. eLife, 8, Article e44652. https://doi.org/10.7554/eLife.44652
Liu, B., Hasrat, Z., Poolman, B., & Boersma, A. J. (2019). Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress. Journal of Bacteriology, 201(10), Article e00708-18. https://doi.org/10.1128/JB.00708-18
Smigiel, W. M., Lefrancois, P., & Poolman, B. (2019). Physicochemical considerations for bottom-up synthetic biology. Emerging topics in life sciences, 3(5), 445-458. Article ETLS20190017. https://doi.org/10.1042/ETLS20190017
Bianchi, F., Van't Klooster, J. S., Ruiz, S. J., & Poolman, B. (2019). Regulation of Amino Acid Transport in Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews, 83(4), Article e00024-19. https://doi.org/10.1128/MMBR.00024-19
Leth, M. L., Ejby, M., Madland, E., Kitaoku, Y., Slotboom, D. J., Guskov, A., Aachmann, F. L., & Abou Hachem, M. (2020). Molecular insight into a new low affinity xylan binding module from the xylanolytic gut symbiont Roseburia intestinalis. The FEBS Journal, 287(10), 2105-2117. https://doi.org/10.1111/febs.15117
Garaeva, A. A., Guskov, A., Slotboom, D. J., & Paulino, C. (2019). A one-gate elevator mechanism for the human neutral amino acid transporter ASCT2. Nature Communications, 10, Article 3427. https://doi.org/10.1038/s41467-019-11363-x
Arkhipova, V. I., Trinco, G., Thijs, E., Jensen, S., Slotboom, D., & Guskov, A. (2019). Binding and transport of D-aspartate by the glutamate transporter homologue GltTk. eLife, 8, Article e45286. https://doi.org/10.7554/eLife.45286
Bousis, S., Setyawati, I., Diamanti, E., Slotboom, D. J., & Hirsch, A. K. H. (2019). Energy-Coupling Factor Transporters as Novel Antimicrobial Targets. Advanced Therapeutics, 2(2), Article 1800066. https://doi.org/10.1002/adtp.201800066
Lolkema, J. S., & Slotboom, D. J. (2019). Models to determine the kinetic mechanisms of ion-coupled transporters. The Journal of General Physiology, 151(3), 369-380. https://doi.org/10.1085/jgp.201812055
Ejby, M., Guskov, A., Pichler, M. J., Zanten, G. C., Schoof, E., Saburi, W., Slotboom, D. J., & Abou Hachem, M. (2019). Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles. Molecular Microbiology, 112(1), 114-130. Article 14257. https://doi.org/10.1111/mmi.14257
Novarina, D., Janssens, G. E., Bokern, K., Schut, T., van Oerle, N. C., Kazemier, H. G., Veenhoff, L. M., & Chang, M. (2020). A genome-wide screen identifies genes that suppress the accumulation of spontaneous mutations in young and aged yeast cells. Aging Cell, 19(2), Article e13084. https://doi.org/10.1111/acel.13084
Rempel, I. L., Crane, M. M., Thaller, D. J., Mishra, A., Jansen, D. P. M., Janssens, G., Popken, P., Akşit, A., Kaeberlein, M., van der Giessen, E., Steen, A., Onck, P. R., Lusk, C. P., & Veenhoff, L. M. (2019). Age-dependent deterioration of nuclear pore assembly in mitotic cells decreases transport dynamics. eLife, 8, Article e48186. https://doi.org/10.7554/eLife.48186
Mouton, S. N., Thaller, D. J., Crane, M. M., Rempel, I. L., Steen, A., Kaeberlein, M., Lusk, C. P., Boersma, A. J., & Veenhoff, L. M. (2019). A physicochemical roadmap of yeast replicative aging. (bioRxiv). BioRxiv. https://doi.org/10.1101/858720
Mishra, A., Sipma, W., Veenhoff, L. M., Van der Giessen, E., & Onck, P. R. (2019). The effect of FG-nup phosphorylation on NPC selectivity: A one-bead-per-amino-acid molecular dynamics study. International Journal of Molecular Sciences, 20(3), Article 596. https://doi.org/10.3390/ijms20030596

2018

Henderson, R. K., Fendler, K., & Poolman, B. (2019). Coupling efficiency of secondary active transporters. Current Opinion in Biotechnology, 58, 62-71. https://doi.org/10.1016/j.copbio.2018.11.005
Marques, W. L., Mans, R., Henderson, R. K., Marella, E. R., Horst, J. T., Hulster, E. D., Poolman, B., Daran, J.-M., Pronk, J. T., Gombert, A. K., & van Maris, A. J. A. (2018). Combined engineering of disaccharide transport and phosphorolysis for enhanced ATP yield from sucrose fermentation in Saccharomyces cerevisiae. Metabolic Engineering, 45, 121-133. https://doi.org/10.1016/j.ymben.2017.11.012
Lycklama A Nijeholt, J. A., Vietrov, R., Schuurman-Wolters, G. K., & Poolman, B. (2018). Energy coupling efficiency in the Type I ABC transporter GlnPQ. Journal of Molecular Biology, 430(6), 853-866. https://doi.org/10.1016/j.jmb.2018.02.001
Schavemaker, P. E., Boersma, A. J., & Poolman, B. (2018). How Important Is Protein Diffusion in Prokaryotes? Frontiers in Molecular Biosciences, 5, Article fmolb.2018.00093. https://doi.org/10.3389/fmolb.2018.00093
Schavemaker, P. E., & Poolman, B. (2018). (Membrane) Protein Production in Context. Trends in Biochemical Sciences, 43(11), 858-868. https://doi.org/10.1016/j.tibs.2018.08.009
Syga, Ł., Spakman, D., Punter, C. M., & Poolman, B. (2018). Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking. Scientific Reports, 8(1), Article 13789. https://doi.org/10.1038/s41598-018-32166-y
Bartelds, R., Nematollahi, M. H., Pols, T., Stuart, M. C. A., Pardakhty, A., Asadikaram, G., & Poolman, B. (2018). Niosomes, an alternative for liposomal delivery. PLoS ONE, 13(4), Article e0194179. https://doi.org/10.1371/journal.pone.0194179
Schuurman-Wolters, G. K., de Boer, M., Pietrzyk, M. K., & Poolman, B. (2018). Protein linkers provide limits on the domain interactions in the ABC importer GlnPQ and determine the rate of transport. Journal of Molecular Biology, 430(8), 1249-1262. Article j.jmb.2018.02.014. https://doi.org/10.1016/j.jmb.2018.02.014
Bianchi, F., Syga, Ł., Moiset, G., Spakman, D., Schavemaker, P. E., Punter, C. M., Seinen, A.-B., van Oijen, A. M., Robinson, A., & Poolman, B. (2018). Steric exclusion and protein conformation determine the localization of plasma membrane transporters. Nature Communications, 9(1), Article 501. https://doi.org/10.1038/s41467-018-02864-2
Liu, B., Mavrova, S. N., van den Berg, J., Kristensen, S. K., Mantovanelli, L., Veenhoff, L. M., Poolman, B., & Boersma, A. J. (2018). The influence of fluorescent protein maturation on FRET measurements in living cells. ACS Sensors, 3(9), 1735-1742. https://doi.org/10.1021/acssensors.8b00473
Rempel, S., Stanek, W. K., & Slotboom, D. J. (2019). ECF-Type ATP-Binding Cassette Transporters. Annual Review of Biochemistry, 88, 551-576. Article annurev-biochem-013118-111705. https://doi.org/10.1146/annurev-biochem-013118-111705
Garaeva, A., Oostergetel, G., Gati, C., Guskov, A., Batista Paulino, C., & Slotboom, D. (2018). Cryo-EM structure of the human neutral amino acid transporter ASCT2. Nature Structural & Molecular Biology, 25, 515-521. https://doi.org/10.1038/s41594-018-0076-y
Rempel, S., Colucci, E., Gier, J.-W. D., Guskov, A., & Slotboom, D. (2018). Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM. Nature Communications, 9(1), Article 3038. https://doi.org/10.1038/s41467-018-05441-9
Luís da Silva Santos Guimarães, J. A., Rempel, S., Mous, S. T. M., Tambascia Pereira, C., ter Beek, J., Gier, J.-W. D., Guskov, A., & Slotboom, D. (2018). Functional and structural characterization of an ECF-type ABC transporter for vitamin B12. eLife, 7, Article e35828. https://doi.org/10.7554/eLife.35828
Hoorens, M. W. H., Fu, H., Duurkens, R. H., Trinco, G., Arkhipova, V., Feringa, B. L., Poelarends, G. J., Slotboom, D. J., & Szymanski, W. (2018). Glutamate Transporter Inhibitors with Photo-Controlled Activity. Advanced Therapeutics, 1(2), Article 1800028. https://doi.org/10.1002/adtp.201800028
Ma, T., Walko, M., Lepoitevin, M., Janot, J.-M., Balanzat, E., Kocer, A., & Balme, S. (2018). Combining Light-Gated and pH-Responsive Nanopore Based on PEG-Spiropyran Functionalization. Advanced Materials Interfaces, 5(2), Article 1701051. https://doi.org/10.1002/admi.201701051

2017

Cozzoli, L., Gjonaj, L., Stuart, M. C. A., Poolman, B., & Roelfes, G. (2018). Responsive DNA G-quadruplex micelles. Chemical communications (Cambridge, England), 54, 260-263. https://doi.org/10.1039/c7cc07899d
Franken, L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova, V. I., Boekema, E., Poolman, B., & Guskov, A. (2017). A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. Nature Communications, 8, Article 722. https://doi.org/10.1038/s41467-017-00718-x
Liu, B., Åberg, C., van Eerden, F. J., Marrink, S., Poolman, B., & Boersma, A. J. (2017). Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding. Biophysical Journal, 112(9), 1929-1939. https://doi.org/10.1016/j.bpj.2017.04.004
Liu, B., Poolman, B., & Boersma, A. J. (2017). Ionic strength sensing in living cells. ACS chemical biology, 12(10), 2510-2514. Article acschembio.7b00348. https://doi.org/10.1021/acschembio.7b00348
Bartelds, R., Barnoud, J., J. Boersma, A., J. Marrink, S., & Poolman, B. (2017). Lipid phase separation in the presence of hydrocarbons in giant unilamellar vesicles. AIMS Biophysics, 4(4), 528-542. https://doi.org/10.3934/biophy.2017.4.528
van den Berg, J., Boersma, A. J., & Poolman, B. (2017). Microorganisms maintain crowding homeostasis. Nature Reviews Microbiology, 15, 309-318. Article nrmicro.2017.17. https://doi.org/10.1038/nrmicro.2017.17
Henderson, R., & Poolman, B. (2017). Proton-solute coupling mechanism of the maltose transporter from Saccharomyces cerevisiae. Scientific Reports, 7(1), 1-12. Article 14375. https://doi.org/10.1038/s41598-017-14438-1
Van Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters, G., Wijma, H. J., Poolman, B., & Maglia, G. (2017). Real-time conformational changes and controlled orientation of native proteins inside a protein nanoreactor. Journal of the American Chemical Society, 139(51), 18640-18646. Article jacs.7b10106. https://doi.org/10.1021/jacs.7b10106
Schavemaker, P. E., Śmigiel, W. M., & Poolman, B. (2017). Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome. eLife, 6, Article eLife.30084. https://doi.org/10.7554/eLife.30084
Poolman, B., & Noireaux, V. (2017). Synthetic Biology: From engineering living systems to the bottom=up construction of synthetic cells. In J. van Doorn, J. Luirink, J. van der Oost, B. Oudega, L. Robertson, H. Smit, P. Willemsen, & C. Biemans (Eds.), Mighty Microbes: the amazing world of microorganisms (1 ed., Vol. 1, pp. 215-217). Microcanon Foundation. https://www.noireauxlab.org/
Arkhipova, V. I., Guskov, A., & Slotboom, D. (2017). Analysis of the quality of crystallographic data and the limitations of structural models. The Journal of General Physiology, 149(12). https://doi.org/10.1085/jgp.201711852
Monjas, L., Swier, L. J. Y. M., Setyawati, I., Slotboom, D. J., & Hirsch, A. K. H. (2017). Dynamic combinatorial chemistry to identify binders of ThiT, an S-component of the energy-coupling factor transporter for thiamine. ChemMedChem, 12(20), 1693-1696. https://doi.org/10.1002/cmdc.201700440
Swier, L. J. Y. M., Monjas, L., Reeßing, F., Oudshoorn, R. C., Aisyah, A., Primke, T., Bakker, M. M., Van Olst, E., Ritschel, T., Faustino, I., Marrink, S. J., Hirsch, A. K. H., & Slotboom, D. J. (2017). Insight into the complete substrate-binding pocket of ThiT by chemical and genetic mutations. MedChemCommun, 8(5), 1121-1130. https://doi.org/10.1039/C7MD00079K
Baumgarten, T., Schlegel, S., Wagner, S., Löw, M., Eriksson, J., Bonde, I., Herrgård, M. J., Heipieper, H. J., Nørholm, M. H. H., Slotboom, D. J., & de Gier, J.-W. (2017). Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3). Scientific Reports, 7, 1-14. Article 45089. https://doi.org/10.1038/srep45089
Jaehme, M., Singh, R., Garaeva, A. A., Duurkens, R. H., & Slotboom, D.-J. (2017). PnuT uses a facilitated diffusion mechanism for thiamine uptake. Journal of general physiology, 150(1), 41-50. Article jgp.201711850. https://doi.org/10.1085/jgp.201711850
Goudsmits, J. M. H., van Oijen, A. M., & Slotboom, D. J. (2017). Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy. Methods in Enzymology, 594, 101-121. https://doi.org/10.1016/bs.mie.2017.05.009
Goudsmits, J. M. H., Slotboom, D. J., & van Oijen, A. M. (2017). Single-molecule visualization of conformational changes and substrate transport in the vitamin B-12 ABC importer BtuCD-F. Nature Communications, 8, Article 1652. https://doi.org/10.1038/s41467-017-01815-7
Lolkema, J., & Slotboom, D. (2017). Structure and elevator mechanism of the Na+-citrate transporter CitS. Current Opinion in Structural Biology, 45, 1-9. https://doi.org/10.1016/j.sbi.2016.10.004
Hjelm, A., Karyolaimos, A., Zhang, Z., Rujas, E., Vikström, D., Slotboom, D. J., & de Gier, J.-W. (2017). Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins. ACS Synthetic Biology, 6(6), 985-994. Article acssynbio.6b00321. https://doi.org/10.1021/acssynbio.6b00321
Lohse, J., Swier, L. J. Y. M., Oudshoorn, R. C., Médard, G., Kuster, B., Slotboom, D. J., & Witte, M. D. (2017). Targeted Diazotransfer Reagents Enable Selective Modification of Proteins with Azides. BIOCONJUGATE CHEMISTRY, 28(4), 913-917. https://doi.org/10.1021/acs.bioconjchem.7b00110
Gati, C., Stetsenko, A., Slotboom, D., Scheres, S., & Guskov, A. (2017). The structural basis of proton driven zinc transport by ZntB. Nature Communications, 8, Article 1313. https://doi.org/10.1038/s41467-017-01483-7
Kuipers, G., Karyolaimos, A., Zhang, Z., Ismail, N., Trinco, G., Vikstrom, D., Slotboom, D. J., & de Gier, J.-W. (2017). The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli. Microbial Cell Factories, 16, Article 226. https://doi.org/10.1186/s12934-017-0840-4
Cabrera, M., Novarina, D., Rempel, I. L., Veenhoff, L. M., & Chang, M. (2017). A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae. Microbial Cell, 4(5), 169-174. https://doi.org/10.15698/mic2017.05.573
Novarina, D., Mavrova, S. N., Janssens, G. E., Rempel, I. L., Veenhoff, L. M., & Chang, M. (2017). Increased genome instability is not accompanied by sensitivity to DNA damaging agents in aged yeast cells. Dna repair, 54, 1-7. https://doi.org/10.1016/j.dnarep.2017.03.005
Calle, D., Yilmaz, D., Cerdan, S., & Kocer, A. (2017). Drug delivery from engineered organisms and nanocarriers as monitored by multimodal imaging technologies. AIMS Bioengineering, 4(2), 198-222. https://doi.org/10.3934/bioeng.2017.2.198
Melo, M. N., Arnarez, C., Sikkema, H., Kumar, N., Walko, M., Berendsen, H. J. C., Kocer, A., Marrink, S. J., & Ingólfsson, H. I. (2017). High-Throughput Simulations Reveal Membrane-Mediated Effects of Alcohols on MscL Gating. Journal of the American Chemical Society, 139(7), 2664-2671. https://doi.org/10.1021/jacs.6b11091
Stetsenko, A., & Guskov, A. (2017). An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization. Crystals, 7(7), 1-16. Article 197. https://doi.org/10.3390/cryst7070197

2016

Scheepers, G. H., Lycklama A Nijeholt, J. A., & Poolman, B. (2016). An updated structural classification of substrate-binding proteins. FEBS Letters, 590(23), 4393-4401. https://doi.org/10.1002/1873-3468.12445
Popov-Čeleketić, D., Bianchi, F., Ruiz, S. J., Meutiawati, F., & Poolman, B. (2016). A Plasma Membrane Association Module in Yeast Amino Acid Transporters. The Journal of Biological Chemistry, 291(31), 16024-16037. https://doi.org/10.1074/jbc.M115.706770
Bianchi, F., Klooster, J. S. V. ., Ruiz, S. J., Luck, K., Pols, T., Urbatsch, I. L., & Poolman, B. (2016). Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae. Scientific Reports, 6, 1-13. Article 31443. https://doi.org/10.1038/srep31443
van den Berg, J., & Poolman, B. (2016). Compartmental Volume Regulation of E. Coli under Various Growth Conditions. Biophysical Journal, 110(3), 35A-35A. https://doi.org/10.1016/j.bpj.2015.11.259
Ruiz, S. J., Schuurman-Wolters, G. K., & Poolman, B. (2016). Crystal Structure of the Substrate-Binding Domain from Listeria monocytogenes Bile-Resistance Determinant BilE. Liquid Crystals, 6(12), 1-16. Article cryst6120162. https://doi.org/10.3390/cryst6120162
Maglia, G., Soskine, M., Biesemans, A., Meervelt, V., & Poolman, B. (2016). Nanopores with internal protein adaptors. (Patent No. WO2016166232). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016166232A1&KC=A1&FT=D&ND=3&date=20161020&DB=&locale=en_EP
van den Berg, J., Galbiati, H., Rasmussen, A., Miller, S., & Poolman, B. (2016). On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli. Scientific Reports, 6, Article 32709 . https://doi.org/10.1038/srep32709
van Gijtenbeek, L. A., Robinson, A., van Oijen , A., Poolman, B., & Kok, J. (2016). On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins. PLoS genetics, 12(12), 1-28. Article e1006523. https://doi.org/10.1371/journal.pgen.1006523
Boersma, A., Liu, B., Åberg, C., & Poolman, B. (2016). Quantification of Macromolecular Crowding in Living Cells. Biophysical Journal, 110(3), 368A-368A. https://doi.org/10.1016/j.bpj.2015.11.1984
Ejby, M., Fredslund, F., Andersen, J. M., Zagar, A. V., Henriksen, J. R., Andersen, T. L., Svensson, B., Slotboom, D. J., & Abou Hachem, M. (2016). An ATP Binding Cassette Transporter Mediates the Uptake of alpha-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates. The Journal of Biological Chemistry, 291(38), 20220-20231. https://doi.org/10.1074/jbc.M116.746529
Guskov, A., Jensen, S., Faustino, I., Marrink, S. J., & Slotboom, D. J. (2016). Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue GltTk. Nature Communications, 7, 1-6. Article 13420. https://doi.org/10.1038/ncomms13420
Monjas , L., Swier, L. J. Y. M., de Voogd, A. R., Oudshoorn, R. C., Hirsch, A. K. H., & Slotboom, D. J. (2016). Design and synthesis of thiamine analogues to study their binding to the ECF transporter for thiamine in bacteria. MedChemCommun, 7(5), 966-971. https://doi.org/10.1039/c6md00022c
Jähme, M., Guskov, A., & Slotboom, D. J. (2016). Pnu Transporters: Ain't They SWEET? Trends in Biochemical Sciences, 41(2), 117-118. https://doi.org/10.1016/j.tibs.2015.11.013
Guskov, A., & Slotboom, D. (2016). Size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS) to determine protein oligomeric states. In E. Pebay-Peyroula, H. Nury, F. Parcy, R. W. H. Ruigrok, C. Ziegler, & L. F. Cugliandolo (Eds.), From molecules to living organisms: an interplay between biology and physics: Lecture Notes of the Les Houches School of Physics: Volume 102, July 2014 (pp. 169-183). (Lecture Notes of the Les Houches School of Physics; Vol. 102). Oxford University Press. https://doi.org/10.1093/acprof:oso/9780198752950.003.0006
Swier, L. J. Y. M., Guskov, A., & Slotboom, D. J. (2016). Structural insight in the toppling mechanism of an energy-coupling factor transporter. Nature Communications, 7, 1-11. Article 11072. https://doi.org/10.1038/ncomms11072
Janssens, G. E., & Veenhoff, L. M. (2016). Evidence for the hallmarks of human aging in replicatively aging yeast. Microbial Cell, 3(7), 263-274. https://doi.org/10.15698/mic2016.07.510
Ghavami, A., Veenhoff, L. M., Van der Giessen, E., & Onck, P. R. (2016). Intrinsically Disordered Proteins: Gatekeepers of the Nuclear Pore Complex. Biophysical Journal, 110(3, suppl. 1), 358A-358A. https://doi.org/10.1016/j.bpj.2015.11.1930
Janssens, G. E., & Veenhoff, L. M. (2016). The Natural Variation in Lifespans of Single Yeast Cells Is Related to Variation in Cell Size, Ribosomal Protein, and Division Time. PLoS ONE, 11(12), Article e0167394. https://doi.org/10.1371/journal.pone.0167394
Dimitrova, A., Walko, M., Shabestari, M. H., Kumar, P., Huber, M., & Kocer, A. (2016). In situ, Reversible Gating of a Mechanosensitive Ion Channel through Protein-Lipid Interactions. Frontiers in Physiology, 7, Article 409. https://doi.org/10.3389/fphys.2016.00409

2015

Fulyani, F., Schuurman-Wolters, G., Slotboom, D.-J., & Poolman, B. (2016). Relative rates of amino acid import via the ABC transporter GlnPQ determine the growth performance of Lactococcus lactis. Journal of Bacteriology, 198(3), 477-485. Article JB.00685-15. https://doi.org/10.1128/JB.00685-15
Swier, L. J. Y. M., Slotboom, D., & Poolman, B. (2015). ABC Importers. In A. M. George (Ed.), ABC Transporters - 40 Years on (1 ed., Vol. 1, pp. 3-36). Springer. https://doi.org/10.1007/978-3-319-23476-2_1
Laba, J. K., Steen, A., Popken, P., Chernova, A., Poolman, B., & Veenhoff, L. M. (2015). Active Nuclear Import of Membrane Proteins Revisited. Cells, 4(4), 653-673. https://doi.org/10.3390/cells4040653
Boersma, A. J., Zuhorn, I. S., & Poolman, B. (2015). A sensor for quantification of macromolecular crowding in living cells. Nature Methods, 12(3), 227-229. https://doi.org/10.1038/nmeth.3257
Gouridis, G., Schuurman-Wolters, G., Ploetz, E., Husada, F., Vietrov, R., de Boer, M., Cordes, T., & Poolman, B. (2015). Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ. Nature Structural & Molecular Biology, 22(1), 57-64. https://doi.org/10.1038/nsmb.2929
Spitzer, J., Pielak, G. J., & Poolman, B. (2015). Emergence of life: Physical chemistry changes the paradigm. Biology Direct, 10(33). https://doi.org/10.1186/s13062-015-0060-y
Ho, F. Y., & Poolman, B. (2015). Engineering Escherichia coli for Functional Expression of Membrane Proteins. Methods in Enzymology, 556, 3-21. https://doi.org/10.1016/bs.mie.2015.01.003
Driessen, A. J. M., & Poolman, B. (2015). In memoriam: Wilhelmus Nicolaas Konings (1937-2014). Extremophiles, 19, 233-234. https://doi.org/10.1007/s00792-015-0736-3
Kralt, A., Carretta, M., Mari, M., Reggiori, F., Steen, A., Poolman, B., & Veenhoff, L. M. (2015). Intrinsically disordered linker and plasma membrane-binding motif sort ist2 and ssy1 to junctions. Traffic, 16(2), 135-147. https://doi.org/10.1111/tra.12243
Goel, A., Eckhardt, T. H., Puri, P., de Jong, A., Branco dos Santos, F., Giera, M., Fusetti, F., de Vos, W. M., Kok, J., Poolman, B., Molenaar, D., Kuipers, O. P., & Teusink, B. (2015). Protein costs do not explain evolution of metabolic strategies and regulation of ribosomal content: does protein investment explain an anaerobic bacterial Crabtree effect? Molecular Microbiology, 97(1), 77-92. https://doi.org/10.1111/mmi.13012
Zollmann, T., Moiset Coll, G., Tumulka, F., Tampé, R., Poolman, B., & Abele, R. (2015). Single liposome analysis of peptide translocation by the ABC transporter TAPL. Proceedings of the National Academy of Sciences of the United States of America, 112(7), 2046-2051. https://doi.org/10.1073/pnas.1418100112
Popken, P., Ghavami, A., Onck, P. R., Poolman, B., & Veenhoff, L. M. (2015). Size-dependent leak of soluble and membrane proteins through the yeast nuclear pore complex. Molecular Biology of the Cell, 26(7), 1386-1394. https://doi.org/10.1091/mbc.E14-07-1175
Husada, F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G. K., Ploetz, E., de Boer, M., Poolman, B., & Cordes, T. (2015). Watching conformational dynamics of ABC transporters with single-molecule tools. Biochemical Society Transactions, 43(5), 1041-1047. https://doi.org/10.1042/BST20150140
Majsnerowska, M., Ter Beek, J., Stanek, W. K., Duurkens, R., & Slotboom, D. J. (2015). Competition between Different S-Components for the Shared Energy Coupling Factor Module in Energy Coupling Factor Transporters. Biochemistry, 54, 4763-4766. https://doi.org/10.1021/acs.biochem.5b00609
Zhang, Z., Kuipers, G., Niemiec, Ł., Baumgarten, T., Slotboom, D. J., de Gier, J.-W., & Hjelm, A. (2015). High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG. Microbial Cell Factories, 14(1), 1-11. Article 142. https://doi.org/10.1186/s12934-015-0328-z
Hänelt, I., Jensen, S., Wunnicke, D., & Slotboom, D. J. (2015). Low affinity and slow Na+-binding precedes high affinity aspartate binding in GltPh. The Journal of Biological Chemistry, 290(26), 15962-15972. https://doi.org/10.1074/jbc.M115.656876
Swier, L. J. Y. M., Monjas, L., Guskov, A., de Voogd, A. R., Erkens, G. B., Slotboom, D. J., & Hirsch, A. K. H. (2015). Structure-Based Design of Potent Small-Molecule Binders to the S-Component of the ECF Transporter for Thiamine. ChemBioChem, 16(5), 819-826. https://doi.org/10.1002/cbic.201402673
Jähme, M., & Slotboom, D. J. (2015). Structure, function, evolution, and application of bacterial Pnu-type vitamin transporters. Biological Chemistry, 396(9-10), 955-966. https://doi.org/10.1515/hsz-2015-0113
Lolkema, J. S., & Slotboom, D.-J. (2015). The Hill analysis and co-ion-driven transporter kinetics. Journal of general physiology, 145(6), 565-574. https://doi.org/10.1085/jgp.201411332
Jähme, M., Guskov, A., & Slotboom, D. (2015). The twisted relation between Pnu and SWEET transporters. Trends in Biochemical Sciences, 40(4), 183-188. https://doi.org/10.1016/j.tibs.2015.02.002
Kralt, A., Jagalur, N. B., van den Boom, V., Lokareddy, R. K., Steen, A., Cingolani, G., Fornerod, M., & Veenhoff, L. M. (2015). Conservation of inner nuclear membrane targeting sequences in mammalian Pom121 and yeast Heh2 membrane proteins. Molecular Biology of the Cell, 26(18), 3301-3312. https://doi.org/10.1091/mbc.E15-03-0184
Lokareddy, R. K., Hapsari, R. A., van Rheenen, M., Pumroy, R. A., Bhardwaj, A., Steen, A., Veenhoff, L. M., & Cingolani, G. (2015). Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure, 23(7), 1305-1316. https://doi.org/10.1016/j.str.2015.04.017
Janssens, G. E., Meinema, A. C., Gonzalez, J., Wolters, J. C., Schmidt, A., Guryev, V., Bischoff, R., Wit, E. C., Veenhoff, L. M., & Heinemann, M. (2015). Protein biogenesis machinery is a driver of replicative aging in yeast. eLife, 4, Article e08527. https://doi.org/10.7554/eLife.08527
Pacheco-Torres, J., Mukherjee, N., Walko, M., Lopez-Larrubia, P., Ballesteros, P., Cerdan, S., & Kocer, A. (2015). Image guided drug release from pH-sensitive Ion channel-functionalized stealth liposomes into an in vivo glioblastoma model. Nanomedicine-Nanotechnology biology and medicine, 11(6), 1345-1354. https://doi.org/10.1016/j.nano.2015.03.014
Kocer, A. (2015). Mechanisms of mechanosensing - mechanosensitive channels, function and re-engineering. Current Opinion in Chemical Biology, 29, 120-127. https://doi.org/10.1016/j.cbpa.2015.10.006
Yilmaz, D., Dimitrova, A. I., Walko, M., & Kocer, A. (2015). Study of light-induced MscL gating by EPR spectroscopy. European Biophysics Journal, 44(7), 557-565. https://doi.org/10.1007/s00249-015-1063-4
Konijnenberg, A., Bannwarth, L., Yilmaz, D., Kocer, A., Venien-Bryan, C., & Sobott, F. (2015). Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment. Protein Science, 24(8), 1292-1300. https://doi.org/10.1002/pro.2703

2014

Jähme, M., & Slotboom, D. J. (2015). Diversity of membrane transport proteins for vitamins in bacteria and archaea. Biochimica et biophysica acta, 1850(3), 565-576. https://doi.org/10.1016/j.bbagen.2014.05.006
Jähme, M., Guskov, A., & Slotboom, D. J. (2014). Crystal structure of the vitamin B3 transporter PnuC, a full-length SWEET homolog. Nature Structural & Molecular Biology, 21(11), 1013-1015. https://doi.org/10.1038/nsmb.2909
Slotboom, D. J. (2014). Structural and mechanistic insights into prokaryotic energy-coupling factor transporters. Nature Reviews Microbiology, 12(2), 79-87. https://doi.org/10.1038/nrmicro3175
ter Beek, J., Guskov, A., & Slotboom, D. J. (2014). Structural diversity of ABC transporters. Journal of general physiology, 143(4), 419-435. https://doi.org/10.1085/jgp.201411164
Ghavami, A., Veenhoff, L. M., van der Giessen, E., & Onck, P. R. (2014). Probing the Disordered Domain of the Nuclear Pore Complex through Coarse-Grained Molecular Dynamics Simulations. Biophysical Journal, 107(6), 1393-1402. https://doi.org/10.1016/j.bpj.2014.07.060
Laba, J. K., Steen, A., & Veenhoff, L. M. (2014). Traffic to the inner membrane of the nuclear envelope. Current Opinion in Cell Biology, 28, 36-45. https://doi.org/10.1016/j.ceb.2014.01.006
Konijnenberg, A., Yilmaz, D., Ingólfsson, H. I., Dimitrova, A., Marrink, S. J., Li, Z., Vénien-Bryan, C., Sobott, F., & Koçer, A. (2014). Global structural changes of an ion channel during its gating are followed by ion mobility mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17170-17175. https://doi.org/10.1073/pnas.1413118111
Urban, M., Kleefen, A., Mukherjee, N., Seelheim, P., Windschiegl, B., Brueggen, M. V. D., Kocer, A., & Tampe, R. (2014). Highly Parallel Transport Recordings on a Membrane-on-Nanopore Chip at Single Molecule Resolution. Nano Letters, 14(3), 1674-1680. http://pubs.acs.org/doi/ipdf/10.1021/nl5002873
Ingólfsson, H. I., Thakur, P., Herold, K. F., Hobart, E. A., Ramsey, N. B., Periole, X., de Jong, D. H., Zwama, M., Yilmaz, D., Hall, K., Maretzky, T., Hemmings, H. C., Blobel, C., Marrink, S. J., Kocer, A., Sack, J. T., & Andersen, O. S. (2014). Phytochemicals Perturb Membranes and Promiscuously Alter Protein Function. ACS chemical biology, 9(8), 1788-1798. https://doi.org/10.1021/cb500086e
Barthmes, M., Jose, M. D. F., Birkner, J. P., Brüggemann, A., Wahl-Schott, C., & Kocer, A. (2014). Studying mechanosensitive ion channels with an automated patch clamp. European Biophysics Journal, 43(2-3), 97-104. https://doi.org/10.1007/s00249-014-0944-2
Mukherjee, N., Jose, M., Birkner, J. P., Walko, M., Ingolfsson, H. I., Dimitrova, A., Arnarez, C., Marrink, S. J., & Kocer, A. (2014). The activation mode of the mechanosensitive ion channel, MscL, by lysophosphatidylcholine differs from tension-induced gating. The FASEB Journal, 28(10), 4292-4302. https://doi.org/10.1096/fj.14-251579

2013

Hänelt, I., Wunnicke, D., Bordignon, E., Steinhoff, H.-J., & Slotboom, D. J. (2013). Conformational heterogeneity of the aspartate transporter Glt(Ph). Nature Structural & Molecular Biology, 20(2), 210-214. https://doi.org/10.1038/nsmb.2471
Jensen, S., Guskov, A., Rempel, S., Hänelt, I., & Slotboom, D. J. (2013). Crystal structure of a substrate-free aspartate transporter. Nature Structural & Molecular Biology, 20(10), 1224-1226. https://doi.org/10.1038/nsmb.2663
Slotboom, D. J. (2013). Flipping the Sidedness of Disulfide Bond Formation. Journal of Molecular Biology, 425(18), 3265-3267. https://doi.org/10.1016/j.jmb.2013.07.005
Fulyani, F., Schuurman-Wolters, G. K., Vujicic - Zagar, A., Guskov, A., Slotboom, D.-J., & Poolman, B. (2013). Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria. Structure, 21(10), 1879-1888. https://doi.org/10.1016/j.str.2013.07.020
Kolbusz, M. A., Slotboom, D. J., & Lolkema, J. S. (2013). Genomic distribution of the small multidrug resistance protein EmrE over 29 Escherichia coli strains reveals two forms of the protein. Febs Journal, 280(1), 244-255. https://doi.org/10.1111/febs.12065
Ejby, M., Fredslund, F., Vujicic - Zagar, A., Svensson, B., Slotboom, D. J., & Abou Hachem, M. (2013). Structural basis for arabinoxylo-oligosaccharide capture by the probiotic Bifidobacterium animalis subsp lactis Bl-04. Molecular Microbiology, 90(5), 1100-1112. https://doi.org/10.1111/mmi.12419
Poolman, B., & Slotboom, D.-J. (2013). Substrate Capture by ABC Transporters. In G. C. K. Roberts (Ed.), Encyclopedia of Biophysics (pp. 2503-2509). Springer.
Majsnerowska, M., Hänelt, I., Wunnicke, D., Schaefer, L. V., Steinhoff, H.-J., & Slotboom, D. J. (2013). Substrate-Induced Conformational Changes in the S-Component ThiT from an Energy Coupling Factor Transporter. Structure, 21(5), 861-867. https://doi.org/10.1016/j.str.2013.03.007
Erkens, G. B., Hänelt, I., Goudsmits, J. M. H., Slotboom, D. J., & van Oijen, A. M. (2013). Unsynchronised subunit motion in single trimeric sodium-coupled aspartate transporters. Nature, 502(7469), 119-123. https://doi.org/10.1038/nature12538
van den Bogaart, G., Meinema, A. C., Krasnikov, V., Veenhoff, L. M., & Poolman, B. (2013). Nuclear transport factor directs localization of protein synthesis during mitosis (Corrigendum; vol 11, pg 350, 2009). Nature Cell Biology, 15(4), 441. https://doi.org/10.1038/ncb2728
Meinema, A. C., Poolman, B., & Veenhoff, L. M. (2013). Quantitative Analysis of Membrane Protein Transport Across the Nuclear Pore Complex. Traffic, 14(5), 487-501. https://doi.org/10.1111/tra.12048
Szymanski, W., Yilmaz, D., Kocer, A., & Feringa, B. L. (2013). Bright Ion Channels and Lipid Bilayers. Accounts of Chemical Research, 46(12), 2910-2923. https://doi.org/10.1021/ar4000357
Mika, J. T., Birkner, J. P., Poolman, B., & Kocer, A. (2013). On the role of individual subunits in MscL gating: "All for one, one for all?". The FASEB Journal, 27(3), 882-892. https://doi.org/10.1096/fj.12-214361
Tauk, L., Thami, T., Ferez, L., Kocer, A., Janot, J.-M., & Dejardin, P. (2013). Thin phosphatidylcholine films as background surfaces with further possibilities of functionalization for biomedical applications. Colloids and Surfaces B: Biointerfaces, 101, 189-195. https://doi.org/10.1016/j.colsurfb.2012.06.028
Persson, M., Gullberg, M., Tolf, C., Lindberg, A. M., Mansson, A., & Kocer, A. (2013). Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments. PLoS ONE, 8(2), Article e55931. https://doi.org/10.1371/journal.pone.0055931
Halza, E., Bro, T. H., Bilenberg, B., & Kocer, A. (2013). Well-Defined Microapertures for Ion Channel Biosensors. Analytical Chemistry, 85(2), 811-815. https://doi.org/10.1021/ac303005g
How, J., Zhang, A., Phillips, M., Reynaud, A., Lu, S. Y., Pan, L. X., Ho, H. T., Yau, Y. H., Guskov, A., Pervushin, K., Shochat, S. G., & Eshaghi, S. (2013). Comprehensive analysis and identification of the human STIM1 domains for structural and functional studies. PLoS ONE, 8(1), Article e53979. https://doi.org/10.1371/journal.pone.0053979
Nordin, N., Guskov, A., Phua, T., Sahaf, N., Xia, Y., Lu, S., Eshaghi, H., & Eshaghi, S. (2013). Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co2+/Mg2+ transport. Biochemical Journal, 451(3), 365-374. https://doi.org/10.1042/BJ20121745
Kesters, D., Thompson, A. J., Brams, M., van Elk, R., Spurny, R., Geitmann, M., Villalgordo, J. M., Guskov, A., Danielson, U. H., Lummis, S. C. R., Smit, A. B., & Ulens, C. (2013). Structural basis of ligand recognition in 5-HT3 receptors. Embo Reports, 14(1), 49-56. https://doi.org/10.1038/embor.2012.189
Kemelbekov, U., Saipov, A., Abdildanova, A., Ospanov, I., Luo, Y., Guskov, A., Saenger, W., Imachova, S., Nasyrova, S., & Pichkhadze, G. (2013). Structure and pharmacological studies of the anaesthetic 1-(3-n-butoxypropyl)-4-benzoyloxypiperidin hydrochloride and its complex with beta-cyclodextrin in solution. NMR and IR-spectroscopy data. Journal of inclusion phenomena and macrocyclic chemistry, 77(1-4), 249-257. https://doi.org/10.1007/s10847-012-0239-0

2012

Erkens, G. B., Dosz-Majsnerowska, M., ter Beek, J., & Slotboom, D. J. (2012). Energy Coupling Factor-Type ABC Transporters for Vitamin Uptake in Prokaryotes. Biochemistry, 51(22), 4390-4396. https://doi.org/10.1021/bi300504v
Schlegel, S., Lofblom, J., Lee, C., Hjelm, A., Klepsch, M., Strous, M., Drew, D., Slotboom, D. J., & de Gier, J.-W. (2012). Optimizing Membrane Protein Overexpression in the Escherichia coli strain Lemo21(DE3). Journal of Molecular Biology, 423(4), 648-659. https://doi.org/10.1016/j.jmb.2012.07.019
Kolbusz, M. A., Slotboom, D. J., & Lolkema, J. S. (2012). Role of Individual Positive Charges in the Membrane Orientation and Activity of Transporters of the Small Multidrug Resistance Family. Biochemistry, 51(44), 8867-8876. https://doi.org/10.1021/bi300854c
Berntsson, R. P. .-A., ter Beek, J., Dosz-Majsnerowska, M., Duurkens, R. H., Puri, P., Poolman, B., & Slotboom, D.-J. (2012). Structural divergence of paralogous S components from ECF-type ABC transporters. Proceedings of the National Academy of Sciences of the United States of America, 109(35), 13990-13995. https://doi.org/10.1073/pnas.1203219109
Berntsson, R. P. .-A., Schuurman-Wolters, G. K., Dunny, G., Slotboom, D.-J., & Poolman, B. (2012). Structure and Mode of Peptide Binding of Pheromone Receptor PrgZ. The Journal of Biological Chemistry, 287(44), 37165-37170. https://doi.org/10.1074/jbc.M112.386334
Meinema, A. C., Poolman, B., & Veenhoff, L. M. (2012). The transport of integral membrane proteins across the nuclear pore complex. Nucleus-Austin, 3(4), 322-329. https://doi.org/10.4161/nucl.20439
Mukherjee, N., & Kocer, A. (2012). Activation of Ms Channel by LPC: A Trigger for MscL Gating in the Absence of Applied Tension. Biophysical Journal, 102(3), 122a-122a. https://doi.org/10.1016/j.bpj.2011.11.681
Birkner, J. P., Poolman, B., & Kocer, A. (2012). Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution. Proceedings of the National Academy of Sciences of the United States of America, 109(32), 12944-12949. https://doi.org/10.1073/pnas.1205270109
Kocer, A., Tauk, L., & Dejardin, P. (2012). Nanopore sensors: From hybrid to abiotic systems. Biosensors and Bioelectronics, 38(1), 1-10. https://doi.org/10.1016/j.bios.2012.05.013
Ferez, L., Thami, T., Tauk, L., Akpalo, E., Flaud, V., Kocer, A., Janot, J., & Dejardin, P. (2012). Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head. In T. Horbett, J. L. Brash, & W. Norde (Eds.), Proteins at Interfaces III (pp. 677-692). Oxford University Press. https://doi.org/10.1021/bk-2012-1120.ch031
Guskov, A., Nordin, N., Reynaud, A., Engman, H., Lundbäck, A.-K., Jong, A. J. O., Cornvik, T., Phua, T., & Eshaghi, S. (2012). Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA. Proceedings of the National Academy of Sciences of the United States of America, 109(45), 18459-18464. https://doi.org/10.1073/pnas.1210076109
Guskov, A., & Eshaghi, S. (2012). The mechanisms of Mg2+ and Co2+ transport by the CorA family of divalent cation transporters. Current topics in membranes, 69, 393-414. https://doi.org/10.1016/B978-0-12-394390-3.00014-8

2011

Klepsch, M. M., Kovermann, M., Löw, C., Balbach, J., Permentier, H. P., Fusetti, F., de Gier, J. W., Gier, J.-W. D., Slotboom, D. J., & Berntsson, R. P. .-A. (2011). Escherichia coli Peptide Binding Protein OppA Has a Preference for Positively Charged Peptides. Journal of Molecular Biology, 414(1), 75-85. https://doi.org/10.1016/j.jmb.2011.09.043
Berntsson, R. P.-A., Thunnissen, A.-M. W. H., Poolman, B., & Slotboom, D.-J. (2011). Importance of a Hydrophobic Pocket for Peptide Binding in Lactococcal OppA. Journal of Bacteriology, 193(16), 4254-4256. https://doi.org/10.1128/JB.00447-11
Steen, A., Wiederhold, E., Gandhi, T., Breitling, R., & Slotboom, D. J. (2011). Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR. Molecular & Cellular Proteomics, 10(7), MCP200–1-MCP200–16. https://doi.org/10.1074/mcp.M000052-MCP200
Beek, J. T., Duurkens, R. H., Erkens, G. B., & Slotboom, D. J. (2011). Quaternary Structure and Functional Unit of Energy Coupling Factor (ECF)-type Transporters. The Journal of Biological Chemistry, 286(7), 5471-5475. https://doi.org/10.1074/jbc.M110.199224
Meinema, A. C., Laba, J. K., Hapsari, R. A., Otten, R., Mulder, F. A. A., Kralt, A., van den Bogaart, G., Lusk, C. P., Poolman, B., & Veenhoff, L. M. (2011). Long Unfolded Linkers Facilitate Membrane Protein Import Through the Nuclear Pore Complex. Science, 333(6038), 90-93. https://doi.org/10.1126/science.1205741
Price, C. E., Kocer, A., Kol, S., van der Berg, J. P., & Driessen, A. J. M. (2011). In vitro synthesis and oligomerization of the mechanosensitive channel of large conductance, MscL, into a functional ion channel. FEBS Letters, 585(1), 249-254. https://doi.org/10.1016/j.febslet.2010.11.057
Kusters, I., Mukherjee, N., de Jong, M. R., Tans, S., Kocer, A., & Driessen, A. J. M. (2011). Taming Membranes: Functional Immobilization of Biological Membranes in Hydrogels. PLoS ONE, 6(5), Article 20435. https://doi.org/10.1371/journal.pone.0020435
Cirac, A. D., Moiset, G., Mika, J. T., Kocer, A., Salvador, P., Poolman, B., Marrink, S. J., & Sengupta, D. (2011). The Molecular Basis for Antimicrobial Activity of Pore-Forming Cyclic Peptides. Biophysical Journal, 100(10), 2422-2431. https://doi.org/10.1016/j.bpj.2011.03.057
Brams, M., Gay, E. A., Sáez, J. C., Guskov, A., van Elk, R., van der Schors, R. C., Peigneur, S., Tytgat, J., Strelkov, S. V., Smit, A. B., Yakel, J. L., & Ulens, C. (2011). Crystal structures of a cysteine-modified mutant in loop D of acetylcholine-binding protein. The Journal of Biological Chemistry, 286(6), 4420-4428. https://doi.org/10.1074/jbc.M110.188730
Kern, J., & Guskov, A. (2011). Lipids in photosystem II: Multifunctional cofactors. Journal of Photochemistry and Photobiology B: Biology, 104(1-2), 19-34. https://doi.org/10.1016/j.jphotobiol.2011.02.025
Broser, M., Glöckner, C., Gabdulkhakov, A., Guskov, A., Buchta, J., Kern, J., Müh, F., Dau, H., Saenger, W., & Zouni, A. (2011). Structural basis of cyanobacterial photosystem II Inhibition by the herbicide terbutryn. The Journal of Biological Chemistry, 286(18), 15964-15972. https://doi.org/10.1074/jbc.M110.215970

2010

Veenhoff, L. M., Meinema, A. C., & Poolman, B. (2010). A karyopherin acts in localized protein synthesis. Cell Cycle, 9(7), 1281-1285. https://doi.org/10.4161/cc.9.7.11110
Mika, J. T., van den Bogaart, G., Veenhoff, L., Krasnikov, V., & Poolman, B. (2010). Molecular sieving properties of the cytoplasm of Escherichia coli and consequences of osmotic stress. Molecular Microbiology, 77(1), 200-207. https://doi.org/10.1111/j.1365-2958.2010.07201.x
Wiederhold, E., Veenhoff, L. M., Poolman, B., & Slotboom, D. J. (2010). Proteomics of Saccharomyces cerevisiae Organelles. Molecular & Cellular Proteomics, 9(3), 431-445. https://doi.org/10.1074/mcp.R900002-MCP200
Kooistra, S. M., van den Boom, V., Thummer, R. P., Johannes, F., Wardenaar, R., Tesson, B. M., Veenhoff, L. M., Fusetti, F., O'Neill, L. P., Turner, B. M., de Haan, G., Eggen, B. J. L., & O’Neill, L. P. (2010). Undifferentiated Embryonic Cell Transcription Factor 1 Regulates ESC Chromatin Organization and Gene Expression. STEM CELLS, 28(10), 1703-1714. https://doi.org/10.1002/stem.497
Broser, M., Gabdulkhakov, A., Kern, J., Guskov, A., Müh, F., Saenger, W., & Zouni, A. (2010). Crystal structure of monomeric photosystem II from Thermosynechococcus elongatus at 3.6-a resolution. The Journal of Biological Chemistry, 285(34), 26255-26262. https://doi.org/10.1074/jbc.M110.127589
Kemelbekov, U. S., Hagenbach, A., Lentz, D., Imachova, S. O., Pichkhadze, G. M., Rustembekov, Z. I., Beketov, K. M., Praliev, K. D., Gabdulkhakov, A., Guskov, A., & Saenger, W. (2010). Pharmacology and structures of the free base of the anaesthetic kazcaine and its complex with beta-cyclodextrin. Journal of inclusion phenomena and macrocyclic chemistry, 68(3-4), 323-330. https://doi.org/10.1007/s10847-010-9791-7
Guskov, A., Gabdulkhakov, A., Broser, M., Glöckner, C., Hellmich, J., Kern, J., Frank, J., Müh, F., Saenger, W., & Zouni, A. (2010). Recent progress in the crystallographic studies of photosystem II. Chemphyschem, 11(6), 1160-1171. https://doi.org/10.1002/cphc.200900901

2009

Kruegel, U., Veenhoff, L. M., Langbein, J., Wiederhold, E., Liesche, J., Friedrich, T., Grimm, B., Martinoia, E., Poolman, B., & Kuehn, C. (2009). Correction to: Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification (vol 20, pg 2497, 2008). Plant Cell, 21(12), 4059-4060. https://doi.org/10.1105/tpc.109.211260
Bogaart, G. V. D., Meinema, A. C., Krasnikov, V., Veenhoff, L. M., & Poolman, B. (2009). Nuclear transport factor directs localization of protein synthesis during mitosis. Nature Cell Biology, 11(3), 350-U269. https://doi.org/10.1038/ncb1844
Gauci, S., Veenhoff, L. M., Heck, A. J. R., & Krijgsveld, J. (2009). Orthogonal Separation Techniques for the Characterization of the Yeast Nuclear Proteome. Journal of Proteome Research, 8(7), 3451-3463. https://doi.org/10.1021/pr9000948
Guskov, A., Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., & Saenger, W. (2009). Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride. Nature Structural & Molecular Biology, 16(3), 334-342. https://doi.org/10.1038/nsmb.1559
Kern, J., Zouni, A., Guskov, A., & Krauss, N. (2009). Lipids in the Structure of Photosystem I, Photosystem II and the Cytochrome b6f Complex. In H. Wada, & N. Murata (Eds.), Lipids in Photosynthesis: Essential and Regulatory Functions (pp. 203-241). (Advances in Photosynthesis and Respiration; Vol. 30). Springer.
Gabdulkhakov, A., Guskov, A., Broser, M., Kern, J., Müh, F., Saenger, W., & Zouni, A. (2009). Probing the accessibility of the Mn(4)Ca cluster in photosystem II: Channels calculation, noble gas derivatization, and cocrystallization with DMSO. Structure, 17(9), 1223-1234. https://doi.org/10.1016/j.str.2009.07.010

2008

Kruegel, U., Veenhoff, L. M., Langbein, J., Wiederhold, E., Liesche, J., Friedrich, T., Grimm, B., Martinoia, E., Poolman, B., Kuehn, C., Krügel, U., & Kühn, C. (2008). Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification. Plant Cell, 20(9), 2497-2513. https://doi.org/10.1105/tpc.108.058271

2007

Alber, F., Dokudovskaya, S., Veenhoff, L. M., Zhang, W., Kipper, J., Devos, D., Suprapto, A., Karni-Schmidt, O., Williams, R., Chait, B. T., Rout, M. P., & Sali, A. (2007). Determining the architectures of macromolecular assemblies. Nature, 450(7170), 683-694. https://doi.org/10.1038/nature06404
Alber, F., Dokudovskaya, S., Veenhoff, L. M., Zhang, W., Kipper, J., Devos, D., Suprapto, A., Karni-Schmidt, O., Williams, R., Chait, B. T., Sali, A., & Rout, M. P. (2007). The molecular architecture of the nuclear pore complex. Nature, 450(7170), 695-701. https://doi.org/10.1038/nature06405
Kocer, A., Walko, M., Feringa, B. L., & Koçer, A. (2007). Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL. Nature protocols, 2(6), 1426-1437. Article 6. https://doi.org/10.1038/nprot.2007.196

2006

Kocer, A., Walko, M., Bulten, E., Halza, E., Feringa, B. L., & Meijberg, W. (2006). Rationally designed chemical modulators convert a bacterial channel protein into a pH-sensory valve. Angewandte Chemie-International Edition, 45(19), 3126-3130. https://doi.org/10.1002/anie.200503403

2005

Kocer, A., Walko, M., Meijberg, W., & Feringa, B. (2005). A light-actuated nanovalve derived from a channel protein. Science, 309(5735), 755-758. https://doi.org/10.1126/science.1114760
Kocer, A., Bulten, E., Walko, M., Feringa, B., Robillard, G., & Meijberg, W. (2005). A sensory valve in liposomal drug delivery systems. Journal of Controlled Release, 101(1-3), 374-376.

2002

Dokudovskaya, S., Veenhoff, L. M., & Rout, M. P. (2002). Cleave to Leave: Structural Insights into the Dynamic Organization of the Nuclear Pore Complex. Molecular Cell, 10(2). https://doi.org/10.1016/S1097-2765(02)00606-8
Montfort, B. A. V., Doeven, M. K., Canas, B., Veenhoff, L. M., Poolman, B., & Robillard, G. T. (2002). Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometry. Biochimica et Biophysica Acta-Bioenergetics, 1555(1-3), 111 - 115. https://doi.org/10.1016/S0005-2728(02)00264-5
Heuberger, E. H. M. L., Veenhoff, L. M., Duurkens, R. H. T., Friesen, R. H. E., & Poolman, B. (2002). Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation. Journal of Molecular Biology, 317(4), 591-600. https://doi.org/10.1006/jmbi.2002.5416
Veenhoff, L. M., Heuberger, E. H. M. L., & Poolman, B. (2002). Quaternary structure and function of transport proteins. Trends in Biochemical Sciences, 27(5), 242 - 249. https://doi.org/10.1016/S0968-0004(02)02077-7
Bozkaya, H., Yurdaydin, C., Bozdayi, M., Kocer Sagiroglu, A., Erkan, O., & Uzunalimoglu, O. (2002). Lamivudine Is Not Effective in the Treatment of Non-Cirrhotic HBeAg (-) Chronic Hepatitis B Patients with Low Level Viremia.

2001

Gunnewijk, M. G. W., van den Bogaard, P. T. C., Veenhoff, L. M., Heuberger, E. H. M. L., de Vos, W. M., Kleerebezem, M., Kuipers, O. P., & Poolman, B. (2001). Hierarchical control versus autoregulation of carbohydrate utilization in bacteria. Journal of Molecular Microbiology and Biotechnology, 3(3), 401 - 413.
Veenhoff, L. M. (2001). Mechanistic aspects and structural organization of a secondary sugar transporter. s.n.
Veenhoff, L. M., Heuberger, E. H. M. L., & Poolman, B. (2001). The lactose transport protein is a cooperative dimer with two sugar translocation pathways. EMBO Journal, 20(12), 3056 - 3062. https://doi.org/10.1093/emboj/20.12.3056

2000

Veenhoff, L. M., Geertsma, E. R., Poolman, B., & Knol, J. (2000). Close Approximation of Putative α-Helices II, IV, VII, X, and XI in the Translocation Pathway of the Lactose Transport Protein of Streptococcus thermophilus. The Journal of Biological Chemistry, 275(31), 23834 - 23840. https://doi.org/10.1074/jbc.M001343200

1999

Spooner, P. J. R., Veenhoff, L. M., Watts, A., & Poolman, B. (1999). Structural information on a membrane transport protein from nuclear magnetic resonance spectroscopy using sequence- selective nitroxide labeling. Biochemistry, 38(30), 9634 - 9639. https://doi.org/10.1021/bi990745l
Veenhoff, L. M., & Poolman, B. (1999). Substrate recognition at the cytoplasmic and extracellular binding site of the lactose transport protein of Streptococcus thermophilus. The Journal of Biological Chemistry, 274(47), 33244 - 33250. https://doi.org/10.1074/jbc.274.47.33244

1998

van Beilen, J. B., Veenhoff, L., & Witholt, B. (1998). Alkane hydroxylase systems in Pseudomonas aeruginosa strains able to grow on n-octane. Studies in Organic Chemistry, 53(C), 211-215. https://doi.org/10.1016/S0165-3253(98)80026-6

1997

Poolman, B., Gunnewijk, M. G. W., Postma, P. W., & Veenhoff, L. M. (1997). Structure-function relationships in the lactose transport protein of Streptococcus thermophilus. The FASEB Journal, 11(9), 1321.
Veenhoff, L. M., & Poolman, B. (1997). Unidirectional reconstitution of a secondary transport protein. The FASEB Journal, 11(9), 1322.

1996

Veenhoff, L., Liang, W.-J., Henderson, P. J. F., Leblanc, G., & Poolman, B. (1996). Unidirectional reconstitution into detergent-destabilized liposomes of the purified lactose transport system of Streptococcus thermophilus. The Journal of Biological Chemistry, 271(26), 15358 - 15366. https://doi.org/10.1074/jbc.271.26.15358
Poolman, B., Knol, J., Veenhoff, L., & Sulter, G. (1996). Unidirectional reconstitution of a secondary transport protein. Progress in Biophysics & Molecular Biology, 65, PC513-PC513.
Last modified:09 June 2023 8.36 p.m.