Skip to ContentSkip to Navigation
About us Latest news News News articles

Bioinformatics to improve shotgun proteomics analyses. Towards an efficient Maldi-MS pipeline

27 June 2011

PhD ceremony: Mr. T.P. Gandhi, 13.15 uur, Doopsgezinde Kerk, Oude Boteringestraat 33, Groningen

Dissertation: Bioinformatics to improve shotgun proteomics analyses. Towards an efficient Maldi-MS pipeline

Promotor(s): prof. B. Poolman, prof. R. Breitling

Faculty: Mathematics and Natural Sciences

 

Proteins play an overwhelmingly dominant role in living organisms as the work-horses of cells. The term proteomics, coined to serve as an analogy to genomics, is often defined as the comprehensive, quantitative study of protein expression and its changes under the influence of biological perturbations. The goal of a typical proteomics experiment is to juxtapose the set of expressed proteins from a living organism under different conditions such as temperature, mutation, nutrient availability, disease vs. healthy. The idea is to understand a biological system by looking at the differences between two or more states, in case of proteomics in the content, state (qualitative), and levels (quantitative) of proteins. Obviously, before any thorough quantitative analysis of differences can be undertaken, unknown proteins expressed in a biological sample must first be identified. Shotgun proteomics is an indispensible tool in high-throughput analysis of proteins in complex biological samples. Accurate peptide identification from liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) forms the cornerstone of such analyses. The work done in this thesis is related to the improvement in the performance of an LC-MALDI-based proteomics pipeline by focusing on several of its components, the outcome of which has resulted into several new algorithms and software solutions.

Last modified:13 March 2020 01.12 a.m.
Share this Facebook LinkedIn
View this page in: Nederlands

More news

  • 01 April 2025

    NSC’s electoral reform plan may have unwanted consequences

    The new voting system, proposed by minister Uitermark, could jeopardize the fundamental principle of proportional representation, says Davide Grossi, Professor of Collective Decision Making and Computation at the University of Groningen

  • 01 April 2025

    ‘AiNed’ National Growth Fund grant for speeding adoption of AI at SMEs

    Professor Ming Cao receives an ‘AiNed’ Growth Fund grant of EUR 2.4 million for research that will contribute to faster adoption of AI at SMEs in the technical industry in the Netherlands.

  • 01 April 2025

    'Diversity leads to better science'

    In addition to her biological research on ageing, Hannah Dugdale also studies disparities relating to diversity in science. Thanks to the latter, she is one of the two 2024 laureates of the Athena Award, an NWO prize for successful and inspiring...