Artificial cofactors: a marriage of homogeneous catalysis with enzymology
PhD ceremony: Mr. J. Jin, 14.45 uur, Academiegebouw, Broerstraat 5, Groningen
Thesis: Artificial cofactors: a marriage of homogeneous catalysis with enzymology
Promotor(s): prof. D. B. Janssen, prof. M.W. Fraaije
Faculty: Mathematics and Natural Sciences
In the evolution of enzymes, cofactors have been incorporated into enzymes to provide diverse activities. The introduction of artificial cofactors, which can alter the activity and selectivity of enzymes, can now greatly expand the repertoire of reactions of enzymes. This thesis describes the preparation and characterization of two artificial metalloproteins based on papain and penicillin G acylase (PA) that catalyze hydrogenation reactions (chapter 2 and 3). In chapter 4 and 5, the reconstitution of two flavoenzymes, eugenol oxidase (EUGO) and vanillyl alcohol oxidase (VAO), has been investigated to help understanding the process of covalent incorporation of FAD into flavoenzymes, which we consider important for designing artificial flavins and their introduction into apo flavoenzymes.
Last modified: | 13 March 2020 01.14 a.m. |
More news
-
18 July 2024
Smart robots to make smaller chips
A robotic arm in a factory that repeatedly executes the same movement: that’s a thing of the past, states Ming Cao. Researchers of the University of Groningen are collaborating with high-tech companies to make production processes more autonomous.
-
17 July 2024
Veni-grants for ten researchers
The Dutch Research Council (NWO) has awarded a Veni grant of up to €320,000 each to ten researchers of the University of Groningen and the UMCG. The Veni grants are designed for outstanding researchers who have recently gained a PhD.
-
15 July 2024
Funding for RUG researchers from National Growth Fund programme Circular Plastics NL
For research on making plastics circular, Professors Patrizio Raffa and Katja Loos together receive about 1.2 million euros from the National Growth Fund programme Circular Plastics NL.